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CDKN2C Antibody (YA3497)

(Synonyms: CDKN2C; CDKN6; Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; p18-INK4c; p18-INK6)
Customer Review

Based on 1 Customer Validation

CDKN2C Antibody (YA3497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2C.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • CDKN2C Antibody (YA3497) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:40

Product Details

Description

CDKN2C Antibody (YA3497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2C.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 18 kDa;Observed band size: 15 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CDKN2C aa1-110/168.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103022

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Western blot analysis of extracts from 293T(lane 2, 20μg), A549(lane 3, 20μg), HeLa(lane 4, 20μg) and MCF-7(lane 5, 20μg) using CDKN2C Antibody (HY-P81003). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB

  • Expression


    Tissue_specificity:Highest levels found in skeletal muscle. Also found in pancreas and heart

  • Subunit

    Heterodimer of p18 with CDK6

  • SwissProt ID

    P42773

  • Gene ID
    1031 [NCBI]
  • Synonyms

    CDKN2C; CDKN6; Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; p18-INK4c; p18-INK6

  • Research Field

    Cell Biology

CDKN2C Antibody (YA3497) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from 293T(lane 2, 20μg), A549(lane 3, 20μg), HeLa(lane 4, 20μg) and MCF-7(lane 5, 20μg) using CDKN2C Antibody (HY-P81003). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDKN2C Antibody (YA3497) Product Image
    1/16
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • CDKN2C Antibody (YA3497) Product Image
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from 293T(lane 2, 20μg), A549(lane 3, 20μg), HeLa(lane 4, 20μg) and MCF-7(lane 5, 20μg) using CDKN2C Antibody (HY-P81003). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • Experimental Validation Results for CDKN2C Antibody (YA3497)
  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from 293T(lane 2, 20μg), A549(lane 3, 20μg), HeLa(lane 4, 20μg) and MCF-7(lane 5, 20μg) using CDKN2C Antibody (HY-P81003). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Pancreas tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using CDKN2C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81003, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDKN2C Antibody (YA3497) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CDKN2C with CDKN2C Antibody (HY-P81003) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2C Antibody (HY-P81003) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDKN2C Antibody (YA3497) Product Image WB
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image IHC-P
  • CDKN2C Antibody (YA3497) Product Image ICC
  • CDKN2C Antibody (YA3497) Product Image ICC