CDK16 Antibody (YA515)
(Synonyms: PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE)
Based on 1 Customer Validation
CDK16 Antibody (YA515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK16.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
CDK16 Antibody (YA515) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK16.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 65-70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 56 kDa
Synthetic peptide corresponding to Human PCTAIRE1.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CDK16 (cyclin-dependent kinase 16, also known as PCTAIRE1/PCTK1) is an atypical member of the cyclin-dependent kinase family that regulates vesicle trafficking, exocytosis, neuronal differentiation, and spermatogenesis through serine/threonine kinase activity[1][2]. Unlike canonical cell-cycle CDKs, CDK16 requires interaction with Cyclin Y (CCNY) and associated 14-3-3 proteins for full activation, establishing a distinct regulatory mechanism within the CDK superfamily[3][4]. Mechanistically, AMPK-mediated phosphorylation of CCNY at Ser326 enhances CCNY-CDK16 complex formation and stimulates CDK16 kinase activity, linking cellular energy sensing to autophagy regulation[5]. Activated CCNY-CDK16 signaling promotes autophagic flux and is required for efficient AMPK-dependent autophagy through pathways involving ULK1 and Beclin1[5]. In disease-associated models, CDK16 contributes to tumor progression by supporting proliferation, survival, migration, and metastatic phenotypes in multiple cancer types, including triple-negative breast cancer and non-small cell lung cancer[6][7]. In triple-negative breast cancer, CDK16 promotes malignancy through phosphorylation of PRC1, supporting tumor growth and metastatic dissemination[6]. Compared with the closely related PFTAIRE kinases CDK14 and CDK15, only CDK16 efficiently stimulates autophagy downstream of CCNY signaling, indicating functional substrate specificity despite shared cyclin interactions[5]. For experimental applications, selective inhibition or genetic suppression of CDK16 has been used to investigate oncogenic signaling, mTOR pathway regulation, autophagy control, and kinase-dependent cellular phenotypes[7][8].
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Subcellular Localization
Cytoplasm; Cytoplasmic vesicle, secretory vesicle; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Synapse, synaptosome
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Expression
Tissue_specificity:Detected in pancreas islets (at protein level) . Detected in brain and pancreas -
Isoforms & Post-Translational Modification
Q00536 has 3 isomers: Q00536-1: 55716 Da (predicted); Q00536-2: 63458 Da (predicted); Q00536-3: 56375 Da (predicted).
Phosphorylation of CDK16 is essential for the binding of CCNY, but also essential for the regulation of CDK16 kinase activity (PubMed:22798068). Phosphorylation of CDK16 is essential for the binding of CCNYl1, but also essential for the regulation of CDK16 kinase activity (By similarity). Ser-146 and Ser-153 are the most critical sites for the binding of CCNYL1 and for modulating CDK16 kinase activity (By similarity). Phosphorylation at Ser-153 inhibits kinase activity (PubMed:22798068) -
Subunit
Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with BRSK2. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1 (By similarity). Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with CCNY; this interaction increases the CDK16 kinase activity (By similarity). Interacts with CCNYL1; this interaction mutually increases the stability of CDK16 and CCNYL1 and increases the kinase activity of CDK16 (By similarity). Interacts with NSF (By similarity)
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SwissProt ID
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Synonyms
PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE
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Research Field
Signal Transduction
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Sun R, et al. Bromodomain-containing protein 2 induces insulin resistance via the mTOR/Akt signaling pathway and an inflammatory response in adipose tissue. Cell Signal. 2017 Jan;30:92-103. [Content Brief]
[2]. Uniprotkb.
[3]. Kohoutova K, et al. Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16. Nat Commun. 2026 Mar 20;17(1):4262. [Content Brief]
[4]. Mikolcevic P, et al. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Mol Cell Biol. 2012 Feb;32(4):868-79. [Content Brief]
[5]. Dohmen M, et al. AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nat Commun. 2020 Feb 25;11(1):1032. [Content Brief]
[6]. Li X, et al. CDK16 promotes the progression and metastasis of triple-negative breast cancer by phosphorylating PRC1. J Exp Clin Cancer Res. 2022 Apr 21;41(1):149. [Content Brief]
[7]. Veeramani C, et al. Lavatera critica, a green leafy vegetable, controls high fat diet induced hepatic lipid accumulation and oxidative stress through the regulation of lipogenesis and lipolysis genes. Biomed Pharmacother. 2017 Dec;96:1349-1357. [Content Brief]
[8]. Ćwiek P, et al. RNA interference screening identifies a novel role for PCTK1/CDK16 in medulloblastoma with c-Myc amplification. Oncotarget. 2015 Jan 1;6(1):116-29. [Content Brief]
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