Fibulin 1 Antibody (YA2831)(PBS only)
(Synonyms: FBLN; FIBL1)Fibulin 1 Antibody (YA2831) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibulin 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Fibulin 1 Antibody (YA2831) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibulin 1.
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Host Rabbit
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 77 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 77 kDa
Recombinant protein of human Fibulin 1 266—402.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from A549(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using Fibulin 1 Antibody (HY-P83086) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
Fibulin 1 is an Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP
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Subcellular Localization
Secreted, extracellular space, extracellular matrix
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Expression
Tissue_specificity:Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells
Induction:Expression increased by estrogen in ovarian cancer cells -
Isoforms & Post-Translational Modification
P23142 has 4 isomers: P23142-1: 77214 Da (predicted); P23142-2: 61552 Da (predicted); P23142-3: 65443 Da (predicted); P23142-4: 74434 Da (predicted).
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Subunit
Homomultimerizes and interacts with various extracellular matrix components such as FN1, LAMA1, LAMA2, NID, ACAN, CSPG2 and type IV collagen. Also interacts with APP and FGB. Interacts with FBLN7 (By similarity). Interacts with CCN3 (PubMed:9927660)
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SwissProt ID
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Synonyms
FBLN; FIBL1
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Research Field
Cardiovascular
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