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  4. TBP Like 1 Antibody (YA3345)

TBP Like 1 Antibody (YA3345)

Cat. No.: HY-P83600
COA User Guide for Antibodies Technical Support

TBP Like 1 Antibody (YA3345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBP Like 1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

TBP Like 1 Antibody (YA3345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBP Like 1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight
Predicted band size: 21 kDa;
Observed band size: 21 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human TBPL1 aa100-150/186.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:200
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:40
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from Hela (lane2(20μg), 293T(lane3(20μg), Mouse testis tissue (lane4(20μg) and Rat testis tissue (lane5(20μg) using TBP Like 1 Antibody (HY-P83600). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

Background
Function:Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box
Subcellular Localization:Cytoplasm; Nucleus
Expression:
Tissue_specificity:Ubiquitously expressed, with highest levels in the testis and ovary
Subunit:Binds TFIIA and TFIIB
Database
Research Field

Epigenetics and Nuclear Signaling

Synonyms
21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBPL1; TLF; TLP; TLP21
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
TBP Like 1 Antibody (YA3345)
Cat. No.:
HY-P83600
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