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TBP Like 1 Antibody (YA3345)

(Synonyms: 21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBPL1; TLF; TLP; TLP21)
Customer Review

Based on 1 Customer Validation

TBP Like 1 Antibody (YA3345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBP Like 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • TBP Like 1 Antibody (YA3345) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:40 1:50-1:100

Product Details

Description

TBP Like 1 Antibody (YA3345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TBP Like 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database

Entrez Gene: 9519 Human

SwissProt: P62380 Human

Immunogen

A synthesized peptide derived from human TBPL1 aa100-150/186.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Western blot analysis of extracts from Hela (lane2(20μg), 293T(lane3(20μg), Mouse testis tissue (lane4(20μg) and Rat testis tissue (lane5(20μg) using TBP Like 1 Antibody (HY-P83600). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Immunohistochemical analysis of paraffin-embedded human pancreas using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    TBP Like 1 is a Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Ubiquitously expressed, with highest levels in the testis and ovary

  • Subunit

    Binds TFIIA and TFIIB

  • SwissProt ID

    P62380

  • Gene ID
    9519 [NCBI]
  • Synonyms

    21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBPL1; TLF; TLP; TLP21

  • Research Field

    Epigenetics and Nuclear Signaling

TBP Like 1 Antibody (YA3345) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), 293T(lane3(20μg), Mouse testis tissue (lane4(20μg) and Rat testis tissue (lane5(20μg) using TBP Like 1 Antibody (HY-P83600). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image
    1/16
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • TBP Like 1 Antibody (YA3345) Product Image
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), 293T(lane3(20μg), Mouse testis tissue (lane4(20μg) and Rat testis tissue (lane5(20μg) using TBP Like 1 Antibody (HY-P83600). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • Experimental Validation Results for TBP Like 1 Antibody (YA3345)
  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), 293T(lane3(20μg), Mouse testis tissue (lane4(20μg) and Rat testis tissue (lane5(20μg) using TBP Like 1 Antibody (HY-P83600). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using TBP Like 1 Antibody (HY-P83600, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TBP Like 1 Antibody (YA3345) Product Image WB
  • TBP Like 1 Antibody (YA3345) Product Image IHC-P
  • TBP Like 1 Antibody (YA3345) Product Image IHC-P
  • TBP Like 1 Antibody (YA3345) Product Image IHC-P
  • TBP Like 1 Antibody (YA3345) Product Image IHC-P
  • TBP Like 1 Antibody (YA3345) Product Image IHC-P
  • TBP Like 1 Antibody (YA3345) Product Image IHC-P