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Arginase-1 Antibody (YA3647)

(Synonyms: arginase 1)
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Based on 1 Customer Validation

Arginase-1 Antibody (YA3647) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Arginase-1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
  • Arginase-1 Antibody (YA3647) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Arginase-1 Antibody (YA3647) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Arginase-1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Purified recombinant fragment of human ARG1 (AA:1-322) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
    Western blot analysis of extracts from Rat liver tissue (lane2, 20μg), HepG2 (lane3, 20μg), HuH-7 (lane4, 20μg), Rat liver tissue (lane5, 30μg) and HepG2 (lane6, 30μg) using Arginase-1 Antibody (HY-P83950). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
    Flow cytometric analysis of 1X106 HepG2 cells labeling Arginase-1 Antibody (HY-P83950, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Arginase-1 is key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure[1][2][3].

  • Subcellular Localization

    Cytoplasm; Cytoplasmic granule

  • Expression


    Tissue_specificity:In the immune system, it was initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMN]) (PubMed:15546957) . It has also been detected in macrophages of mycobacterial granulomas (PubMed:23749634) . During lung disease, it is expressed in type 2 innate lymphocytes (ILC2) (PubMed:27043409) .

    Induction:By arginine or homoarginine

  • Isoforms & Post-Translational Modification

    P05089 has 3 isomers: P05089-1: 34735 Da (predicted); P05089-2: 35664 Da (predicted); P05089-3: 25356 Da (predicted).

  • Subunit

    Homotrimer (PubMed:16141327, PubMed:17469833, PubMed:17562323, PubMed:18802628, PubMed:2241902). Interacts with CMTM6 (PubMed:28813417)

  • SwissProt ID

    P05089

  • Gene ID
    383 [NCBI]
  • Synonyms

    arginase 1

Arginase-1 Antibody (YA3647) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat liver tissue (lane2, 20μg), HepG2 (lane3, 20μg), HuH-7 (lane4, 20μg), Rat liver tissue (lane5, 30μg) and HepG2 (lane6, 30μg) using Arginase-1 Antibody (HY-P83950). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HepG2 cells labeling Arginase-1 Antibody (HY-P83950, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Arginase-1 Antibody (YA3647) Product Image
    1/16
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
  • Arginase-1 Antibody (YA3647) Product Image
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat liver tissue (lane2, 20μg), HepG2 (lane3, 20μg), HuH-7 (lane4, 20μg), Rat liver tissue (lane5, 30μg) and HepG2 (lane6, 30μg) using Arginase-1 Antibody (HY-P83950). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HepG2 cells labeling Arginase-1 Antibody (HY-P83950, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
  • Experimental Validation Results for Arginase-1 Antibody (YA3647)
  • Arginase-1 Antibody (YA3647) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat liver tissue (lane2, 20μg), HepG2 (lane3, 20μg), HuH-7 (lane4, 20μg), Rat liver tissue (lane5, 30μg) and HepG2 (lane6, 30μg) using Arginase-1 Antibody (HY-P83950). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Arginase-1 Antibody (YA3647) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HepG2 cells labeling Arginase-1 Antibody (HY-P83950, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Arginase-1 Antibody (YA3647) Product Image WB
  • Arginase-1 Antibody (YA3647) Product Image FC