Transketolase Antibody (YA6820)

Cat. No.: HY-P87127: Data Sheet COA
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Transketolase Antibody (YA6820) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transketolase.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Transketolase Antibody (YA6820) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transketolase.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P29401

Gene ID

7086 [NCBI]

Immunogen

Synthetic peptide within human Transketolase aa 41-90.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:30000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
FC FC: Flow Cytometry	1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Rat liver (lane 4(20μg)) using Transketolase Antibody (HY-P87127) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/30000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate

Isoforms & Post-Translational Modification：P29401 has two isomers: P29401-1: 67878 Da (predicted); P29401-2: 68814 Da (predicted).

Subunit：Homodimer

Synonyms

EC 2.2.1.1 antibody; FLJ34765 antibody; TK antibody; TKT 1 antibody; tkt antibody; TKT_HUMAN antibody; TKT1 antibody; Transketolase (Wernicke Korsakoff syndrome) antibody; Transketolase antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)