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Transketolase Antibody (YA6820)

(Synonyms: EC 2.2.1.1 antibody; FLJ34765 antibody; TK antibody; TKT 1 antibody; tkt antibody; TKT_HUMAN antibody; TKT1 antibody; Transketolase (Wernicke Korsakoff syndrome) antibody; Transketolase antibody; )
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Based on 1 Customer Validation

Transketolase Antibody (YA6820) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transketolase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Transketolase Antibody (YA6820)
  • Transketolase Antibody (YA6820) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:30000 1:1000 1:1000 1:2000

Product Details

Description

Transketolase Antibody (YA6820) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transketolase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Immunogen

Synthetic peptide within human Transketolase aa 41-90.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Transketolase Antibody (YA6820)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Rat liver (lane 4(20μg)) using Transketolase Antibody (HY-P87127) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/30000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Transketolase is a key rate-limiting enzyme in the non-oxidative branch of the pentose phosphate pathway (PPP) . Transketolase is critical for energy transduction and for generating ribose for nucleic acid synthesis. Transketolase can convert ribulose 5-phosphate back into glucose 6-phosphate to generate more NADPH, and the reaction is thiamine-dependent. Transketolase has three isomer: TKT, TKTL1 and TKTL2. TKT is expressed in all investigated organisms, and is up-regulated in multiple cancer types[1][2].

  • Isoforms & Post-Translational Modification

    P29401 has two isomers: P29401-1: 67878 Da (predicted); P29401-2: 68814 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P29401

  • Gene ID
    7086 [NCBI]
  • Synonyms

    EC 2.2.1.1 antibody; FLJ34765 antibody; TK antibody; TKT 1 antibody; tkt antibody; TKT_HUMAN antibody; TKT1 antibody; Transketolase (Wernicke Korsakoff syndrome) antibody; Transketolase antibody;

References

[1]. Alexander-Kaufman K, et al. Transketolase: observations in alcohol-related brain damage research. Int J Biochem Cell Biol. 2009 Apr;41(4):717-20. [Content Brief]

[2]. Zhao J, et al. A review on research progress of transketolase. Neurosci Bull. 2009 Apr;25(2):94-9. [Content Brief]

Transketolase Antibody (YA6820) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Rat liver (lane 4(20μg)) using Transketolase Antibody (HY-P87127) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/30000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Transketolase Antibody (YA6820) Product Image
    1/16
  • Experimental Validation Results for Transketolase Antibody (YA6820)
  • Transketolase Antibody (YA6820) Product Image
  • Experimental Validation Results for Transketolase Antibody (YA6820)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Rat liver (lane 4(20μg)) using Transketolase Antibody (HY-P87127) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/30000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Transketolase Antibody (YA6820)
  • Transketolase Antibody (YA6820) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Rat liver (lane 4(20μg)) using Transketolase Antibody (HY-P87127) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/30000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Transketolase Antibody (YA6820) Product Image WB