2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. WDR77 Antibody (YA5281)

WDR77 Antibody (YA5281) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to WDR77.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

WDR77 Antibody (YA5281) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to WDR77.
Host

Mouse
Clonality

Monoclonal
分子量
Observed band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Purified recombinant human WDR77 protein fragments expressed in E.coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100
純度 affinity chromatography. Conjugation Non-conjugated
Modification Unmodified  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334)
Subcellular Localization：Nucleus; Cytoplasm
Expression：
Tissue_specificity:It is highly expressed in the heart, skeletal muscle, spleen, testes, uterus, prostate, and thymus. In the testes, it is expressed in germ cells and interstitial cells, but not in peritubular muscle cells and Sertoli cells. It is expressed in prostate cancer, seminoma, and interstitial cell tumors.
Isoforms & Post-Translational Modification：Q9BQA1 has 2 isomers: Q9BQA1-1: 36724 Da (predicted); Q9BQA1-2: 29651 Da (predicted).
Subunit：Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (PubMed:18984161, PubMed:21081503). Found in a complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (PubMed:21081503). Found in a complex with the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH (PubMed:25284789). Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE (PubMed:11756452, PubMed:33376131). Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/H2AC20, but not with histones H2B, H3 nor H4 (PubMed:16712789). Interacts with CTDP1 and LSM11 (PubMed:12560496, PubMed:16087681). Interacts with APEX1, AR and NKX3-1 (PubMed:12972618, PubMed:19188445). Interacts with CHTOP (PubMed:25284789). Interacts with FAM47E. Interacts with TSC22D2 (PubMed:27337956)
Synonyms
2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77; WD repeat-containing protein 77; WDR77.
ドキュメンテーション
User Guide for Antibodies (1077 KB)

WDR77 Antibody (YA5281) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
WDR77 Antibody (YA5281)
製品番号:
HY-P85589
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