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Cat. No.: HY-P85589

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WDR77 Antibody (YA5281)

Name: WDR77 Antibody (YA5281)
Brand: MedChemExpress
SKU: HY-P85589
Availability: PreOrder

(Synonyms: 2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77; WD repeat-containing protein 77; WDR77.)

WDR77 Antibody (YA5281) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to WDR77.

For research use only. We do not sell to patients.

Host:
Mouse
Application:
WB, ICC/IF
Reactivity :
Human, Mouse, Rat

Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Conjugation:
Non-conjugated

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Storage & Stability
- Shipping
Background
Documentation
Help & FAQs
Classifications
Bioz

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence
Dilution Ratio	1:1000	1:100

Product Details

Description

WDR77 Antibody (YA5281) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to WDR77.

Host Mouse
Clonality Monoclonal
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Immunogen

Purified recombinant human WDR77 protein fragments expressed in E.coli.

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Background

Function

WDR77 is a Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage^[1]^[2].
Subcellular Localization

Nucleus; Cytoplasm
Expression

Tissue_specificity:It is highly expressed in the heart, skeletal muscle, spleen, testes, uterus, prostate, and thymus. In the testes, it is expressed in germ cells and interstitial cells, but not in peritubular muscle cells and Sertoli cells. It is expressed in prostate cancer, seminoma, and interstitial cell tumors.
Isoforms & Post-Translational Modification

Q9BQA1 has 2 isomers: Q9BQA1-1: 36724 Da (predicted); Q9BQA1-2: 29651 Da (predicted).
Subunit

Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (PubMed:18984161, PubMed:21081503). Found in a complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (PubMed:21081503). Found in a complex with the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH (PubMed:25284789). Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE (PubMed:11756452, PubMed:33376131). Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/H2AC20, but not with histones H2B, H3 nor H4 (PubMed:16712789). Interacts with CTDP1 and LSM11 (PubMed:12560496, PubMed:16087681). Interacts with APEX1, AR and NKX3-1 (PubMed:12972618, PubMed:19188445). Interacts with CHTOP (PubMed:25284789). Interacts with FAM47E. Interacts with TSC22D2 (PubMed:27337956)
SwissProt ID

Q9BQA1
Gene ID

79084 [NCBI]
Synonyms

2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77; WD repeat-containing protein 77; WDR77.