Cdc25C Antibody (YA3942)(PBS only)

Cat. No.: HY-P84245A: Data Sheet User Guide for Antibodies
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Cdc25C Antibody (YA3942) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cdc25C.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cdc25C Antibody (YA3942) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cdc25C.

Host

Mouse

Molecular Weight

Predicted band size: 53 kDa;

Observed band size: 58 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P30307

Gene ID

995 [NCBI]

Immunogen

Purified recombinant fragment of human CDC25C aa 1-200.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945)

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P30307 has 5 isomers: P30307-1: 53365 Da (predicted); P30307-2: 50122 Da (predicted); P30307-5: Da (predicted); P30307-3: 48851 Da (predicted); P30307-4: 45608 Da (predicted).
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity

Subunit：Interacts with MAPK14 and 14-3-3 proteins (PubMed:11333986). When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1 (PubMed:12595692). Interacts with MARK3/C-TAK1 (PubMed:12941695)

Synonyms

CDC25; CDC25C

Documentation

User Guide for Antibodies (1077 KB)