FOXP3 Antibody (YA759)
(Synonyms: FOXP3; IPEX; JM2; Forkhead box protein P3; Scurfin)
Based on 1 Customer Validation
FOXP3 Antibody (YA759) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXP3.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, mIHC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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mIHC
mIHC: Multiplex Immunohistochemical
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:1000 | 1:1000 |
Product Details
FOXP3 Antibody (YA759) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXP3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
Entrez Gene: 50943 Human ; 20371 Mouse ; 317382 Rat
SwissProt: Q9BZS1 Human ; Q99JB6 Mouse ;
OMIM: 304790 Human
Synthetic peptide corresponding to Human FOXP3.The exact sequence is proprietary to MCE.
Transfected
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis was performed on extracts from CHO-K1(Flag-FOXP3) (lane 1, 15 μg) using FOXP3 Mouse (7H9) mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C. -
Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using FOXP3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. -
Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using FOXP3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium. -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using FOXP3 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium. -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using FOXP3 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium. -
Immunocytochemistry analysis of Hela cells labeling FOXP3 with FOXP3 Antibody (HY-P80678) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FOXP3 Antibody (HY-P80678) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of MCF-7 cells labeling FOXP3 with FOXP3 Antibody (HY-P80678) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FOXP3 Antibody (HY-P80678) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
FOXP3, so called scurfin, belongs to the forkhead box protein P (FOXP) family. FOXP3 is critically involved in differentiation and function of regulatory T cells or CD4+/CD25+ regulatory T (Treg) cells for cancer immunotherapy. FOXP3 modulates Treg development and functions by immune evasion of tumor cells through imbalance of immunoediting and immunosurveillance in some cancers. FOXP3 is associated with IL-17, RUNX1, STAT3, NF-κB, FOXO3 and other cofactors such as EOS (Ikzf4), interferon regulatory factor 4 (IRF4), special AT-rich sequence-binding protein-1 (SATB1) and GATA1.
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Subcellular Localization
Nucleus,Cytoplasm
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Expression
Induction: Down-regulated in regulatory T-cells (Treg) during inflammation. Up-regulated by FOXO3
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Isoforms & Post-Translational Modification
FOXP3 has four isomers: Q9BZS1-1: 47244 Da (predicted); Q9BZS1-2: 43410 Da (predicted); Q9BZS1-3: 49843 Da (predicted); Q9BZS1-4: 44407 Da (predicted).
Polyubiquitinated, leading to its proteasomal degradation in regulatory T-cells (Treg) which is mediated by STUB1 in a HSPA1A/B-dependent manner. -
Subunit
Homodimer
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SwissProt ID
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Synonyms
FOXP3; IPEX; JM2; Forkhead box protein P3; Scurfin
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Research Field
Cell Biology
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Kim JH, et al. Molecular networks of FOXP family: dual biologic functions, interplay with other molecules and clinical implications in cancer progression. Mol Cancer. 2019 Dec 9;18(1):180. [Content Brief]
[2]. Co M, et al. FOXP transcription factors in vertebrate brain development, function, and disorders. Wiley Interdiscip Rev Dev Biol. 2020 Sep;9(5):e375. [Content Brief]
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