Leukotriene A4 Hydrolase Antibody (YA2400)

Cat. No.: HY-P82655: Data Sheet COA
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Leukotriene A4 Hydrolase Antibody (YA2400) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Leukotriene A4 Hydrolase.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Leukotriene A4 Hydrolase Antibody (YA2400) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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References

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Leukotriene A4 Hydrolase Antibody (YA2400) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Leukotriene A4 Hydrolase.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 69 kDa;

Observed band size: 69 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P09960

Gene ID

4048 [NCBI]

Immunogen

A synthesized peptide derived from human LTA4H aa550-611/611.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P FC

Western blot analysis of extracts from A549 (lane 2(20μg), A549 (lane 3(40μg), using Leukotriene A4 Hydrolase Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Leukotriene A4 Hydrolase Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Leukotriene A4 Hydrolase Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of 1X10^6 Jurkat cells labeling Leukotriene A4 Hydrolase Antibody (YA2400) (HY-P82655, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4) (PubMed:11917124, PubMed:12207002, PubMed:15078870, PubMed:18804029, PubMed:1897988, PubMed:1975494, PubMed:2244921). Also has aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (PubMed:18804029, PubMed:20813919). In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed:20813919, PubMed:24591641). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (PubMed:21206090)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts

Isoforms & Post-Translational Modification：P09960 has 4 isomers: P09960-1: 69285 Da (predicted); P09960-2: 59733 Da (predicted); P09960-3: 57300 Da (predicted); P09960-4: 66852 Da (predicted).
Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity

Subunit：Monomer

RRID

AB_3104755

Database

Entrez Gene: 4048 Human ; 16993 Mouse ; 299732 Rat

SwissProt: P09960 Human ; P24527 Mouse ; P30349 Rat

OMIM: 151570 Human

Research Field

Immunology

Synonyms

LTA4; LTA4 hydrolase; LTA4H

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hl-60-cell.html [Content Brief]

Leukotriene A4 Hydrolase Antibody (YA2400) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Leukotriene A4 Hydrolase Antibody (YA2400)

Leukotriene A4 Hydrolase Antibody (YA2400) Featured Recommendations:

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Leukotriene A4 Hydrolase Antibody (YA2400) Related Classifications

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