Integrin Linked ILK Antibody (YA3111)(PBS only)

Cat. No.: HY-P83366A: Data Sheet User Guide for Antibodies
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Integrin Linked ILK Antibody (YA3111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin Linked ILK.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

Integrin Linked ILK Antibody (YA3111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin Linked ILK.

Host

Rabbit

분자량

Predicted band size: 51 kDa;

Observed band size: 51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13418

Gene ID

3611 [NCBI]

Immunogen

A synthetic peptide of human Integrin linked ILK

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration (PubMed:17420447, PubMed:20005845, PubMed:30367047, PubMed:32528174). Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation (By similarity). Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed:30367047). Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion (PubMed:20005845). Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes (PubMed:16517730). Acts as an upstream effector of both AKT1/PKB and GSK3 (PubMed:9736715). Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA (PubMed:18283114). Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus (PubMed:17420447)

Subcellular Localization：Cell junction, focal adhesion; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium; Cytoplasm, myofibril, sarcomere; Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cell cortex

Expression：
Tissue_specificity:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver

Isoforms & Post-Translational Modification：Q13418 has 3 isomers: Q13418-1: 51419 Da (predicted); Q13418-2: 44349 Da (predicted); Q13418-3: 36467 Da (predicted).
Phosphorylation by PAK1 modulates ILK subcellular location by promoting its nuclear export

Subunit：Component of the heterotrimeric IPP (ILK-PINCH-PARVIN) complex composed of ILK, LIMS1/PINCH and PARVA; the complex binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling (PubMed:11331308, PubMed:12167643, PubMed:12432066, PubMed:19074270, PubMed:19117955, PubMed:21524996, PubMed:30367047, PubMed:35259013). Formation of the IPP complex is dependent on protein kinase C and precedes integrin-mediated cell adhesion and spreading (PubMed:12432066). ILK also interacts with LIMS2/PINCH2 and with PARVB and PARVG which may substitute for LIMS1 and PARVA in the IPP complex; PARVA and PARVB compete for the same binding site (PubMed:11402068, PubMed:12167643, PubMed:15284246, PubMed:15817463, PubMed:16517730, PubMed:20005845, PubMed:20117114). Interaction with PARVG promotes the establishment of cell polarity required for leukocyte migration (PubMed:16517730). Interacts with the cytoplasmic domain of integrin ITGB1 and may also interact with integrins ITGB2, ITGB3 and/or ITGB5 (PubMed:8538749). Interacts probably also with TGFB1I1 (By similarity). Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (PubMed:19118217). Interacts with FERMT2 (By similarity). Interacts with LIMD2; leading to activate the protein kinase activity (PubMed:24590809). Interacts with PXN/PAXILLIN (via LD motif 4) (PubMed:15817463). Interacts with CCDC25 (via cytoplasmic region); initiating the ILK-PARVB cascade to induce cytoskeleton rearrangement and directional migration of cells (PubMed:32528174). Interacts with IQGAP1; the interaction is required for localization of IQGAP1 to the cell cortex (By similarity)

Research Field

Signal Transduction

Synonyms

ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK

각종 서류

User Guide for Antibodies (1077 KB)