HTRA1 Antibody (YA7239)

Cat. No.: HY-P87555: Data Sheet User Guide for Antibodies
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HTRA1 Antibody (YA7239) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HTRA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HTRA1 Antibody (YA7239) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HTRA1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 51 kDa;

Observed band size: 51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q92743

Immunogen

A synthesized peptide derived from human HTRA1.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1000
FC FC: Flow Cytometry	1:200-2000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets

Subcellular Localization：Cell membrane,Secreted,Cytoplasm, cytosol

Expression：
Tissue_Specificity: Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up-regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level)

Isoforms & Post-Translational Modification：Q92743: 480 amino acids, molecular weight 51287 Da.

Subunit：Forms homotrimers

Synonyms

HTRA, PRSS11, HTRA1, Serine protease HTRA1, High-temperature requirement A serine peptidase 1, L56, Serine protease 11

Documentation

User Guide for Antibodies (1077 KB)