DNA Polymerase delta 3 Antibody
(Synonyms: KIAA0039, POLD3, DNA polymerase delta subunit 3, DNA polymerase delta subunit C, DNA polymerase delta subunit p66, DNA polymerase delta subunit p68)DNA Polymerase delta 3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to DNA Polymerase delta 3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
DNA Polymerase delta 3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to DNA Polymerase delta 3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 51 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 51 kDa
Synthetic peptide corresponding to the C-term region of human DNA Polymerase delta 3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
DNA Polymerase delta 3 is an Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the tetrameric DNA polymerase zeta complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the DNA polymerase zeta complex compared to the minimal zeta complex, consisting of only REV3L and REV7[1][2][3][4][5][6][7][8][9][10][11][12][13][14].
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Subcellular Localization
Cytoplasm; Nucleus
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Isoforms & Post-Translational Modification
DNA Polymerase delta 3 has 3 isoforms, Q15054-1: amino acid length is 466, molecular weight is 51400 Da (predicted); Q15054-2: amino acid length is 427, molecular weight is 46801 Da (predicted); Q15054-3: amino acid length is 360, molecular weight is 39320 Da (predicted).
Ubiquitinated, but not targeted to the proteasome. Sumoylated. Sumoylation with SUMO3 may be predominant. -
Subunit
Component of both the DNA polymerase delta and DNA polymerase zeta complexes.
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SwissProt ID
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Synonyms
KIAA0039, POLD3, DNA polymerase delta subunit 3, DNA polymerase delta subunit C, DNA polymerase delta subunit p66, DNA polymerase delta subunit p68
Documentation
References
[1]. Zhang S, et al. A novel DNA damage response: rapid degradation of the p12 subunit of dna polymerase delta. J Biol Chem. 2007 May 25;282(21):15330-40. [Content Brief]
[2]. Chea J, et al. Spatiotemporal recruitment of human DNA polymerase delta to sites of UV damage. Cell Cycle. 2012 Aug 1;11(15):2885-95. [Content Brief]
[3]. Lee YS, et al. Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass. Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):2954-9. [Content Brief]
[4]. Hughes P, et al. Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts. Nucleic Acids Res. 1999 May 15;27(10):2108-14. [Content Brief]
[5]. Mo J, et al. Evidence that DNA polymerase delta isolated by immunoaffinity chromatography exhibits high-molecular weight characteristics and is associated with the KIAA0039 protein and RPA. Biochemistry. 2000 Jun 20;39(24):7245-54. [Content Brief]
[6]. Ducoux M, et al. Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta. J Biol Chem. 2001 Dec 28;276(52):49258-66. [Content Brief]
[7]. Li H, et al. Functional roles of p12, the fourth subunit of human DNA polymerase delta. J Biol Chem. 2006 May 26;281(21):14748-55. [Content Brief]
[8]. Lin SH, et al. Dynamics of enzymatic interactions during short flap human Okazaki fragment processing by two forms of human DNA polymerase δ. DNA Repair (Amst). 2013 Nov;12(11):922-35. [Content Brief]
[9]. Meng X, et al. DNA damage alters DNA polymerase delta to a form that exhibits increased discrimination against modified template bases and mismatched primers. Nucleic Acids Res. 2009 Feb;37(2):647-57. [Content Brief]
[10]. Meng X, et al. The p12 subunit of human polymerase delta modulates the rate and fidelity of DNA synthesis. Biochemistry. 2010 May 4;49(17):3545-54. [Content Brief]
[11]. Ogi T, et al. Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells. Mol Cell. 2010 Mar 12;37(5):714-27. [Content Brief]
[12]. Costantino L, et al. Break-induced replication repair of damaged forks induces genomic duplications in human cells. Science. 2014 Jan 3;343(6166):88-91. [Content Brief]
[13]. Hirota K, et al. The POLD3 subunit of DNA polymerase δ can promote translesion synthesis independently of DNA polymerase ζ. Nucleic Acids Res. 2015 Feb 18;43(3):1671-83. [Content Brief]
[14]. Hirota K, et al. In vivo evidence for translesion synthesis by the replicative DNA polymerase δ. Nucleic Acids Res. 2016 Sep 6;44(15):7242-50. [Content Brief]
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