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Neuropilin-1 Antibody (YA3507)

(Synonyms: NRP1; NP1; NRP; BDCA4; CD304; VEGF165R)
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Based on 1 Customer Validation

Neuropilin-1 Antibody (YA3507) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neuropilin-1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Neuropilin-1 Antibody (YA3507)
  • Neuropilin-1 Antibody (YA3507) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

Neuropilin-1 Antibody (YA3507) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neuropilin-1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 103 kDa
Immunogen

Synthesized peptide of human Neuropilin-1 (AA: 45-59).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718771

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Neuropilin-1 Antibody (YA3507)
    Western blot analysis of extracts from U87 (lane 2(20μg), A549 (lane 3(20μg), MDA-MB-231 (lane 4(20μg), HUVEC (lane 5(20μg) using Neuropilin-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Neuropilin-1 is a Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR; (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection; Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity[1][2][3][4][5][6][7].

  • Subcellular Localization

    Secreted; Mitochondrion membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Expression of isoforms 1 and 2 appears to have no overlap. Expressed in olfactory epithelium (protein level) (PubMed:33082293) . Expressed in fibroblasts (protein level) (PubMed:36213313) . Expressed in blood vessels of various tissues. In developing embryos, primarily present in the nervous system. In adult tissues, highly expressed in the heart and placenta; moderately expressed in the lungs, liver, skeletal muscle, kidneys, and pancreas; and lowly expressed in the adult brain (PubMed:10688880, PubMed:9529250) . Expressed in the central nervous system, including olfactory-related regions such as the olfactory tubercle and paraolfactory gyrus (PubMed:33082293) ; expression of isoforms 1 and 2 appears to have no overlap. Present in hepatocytes of the liver and distal and proximal convoluted tubules of the kidneys.

  • Isoforms & Post-Translational Modification

    O14786 has 3 isomers: O14786-1: 103134 Da (predicted); O14786-2: 71935 Da (predicted); O14786-3: 68376 Da (predicted).

  • Subunit

    Homodimer, and heterodimer with NRP2 (PubMed:17989695). Interacts with FER (By similarity). Interacts with PLXNB1 (PubMed:10520995). Interacts with VEGFA (PubMed:19805273, PubMed:26503042). Interacts with ABCB8/MITOSUR in mitochondria (PubMed:30623799)

  • SwissProt ID

    O14786

  • Gene ID
    8829 [NCBI]
  • Synonyms

    NRP1; NP1; NRP; BDCA4; CD304; VEGF165R

Neuropilin-1 Antibody (YA3507) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U87 (lane 2(20μg), A549 (lane 3(20μg), MDA-MB-231 (lane 4(20μg), HUVEC (lane 5(20μg) using Neuropilin-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Neuropilin-1 Antibody (YA3507) Product Image
    1/16
  • Experimental Validation Results for Neuropilin-1 Antibody (YA3507)
  • Neuropilin-1 Antibody (YA3507) Product Image
  • Experimental Validation Results for Neuropilin-1 Antibody (YA3507)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U87 (lane 2(20μg), A549 (lane 3(20μg), MDA-MB-231 (lane 4(20μg), HUVEC (lane 5(20μg) using Neuropilin-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Neuropilin-1 Antibody (YA3507)
  • Neuropilin-1 Antibody (YA3507) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U87 (lane 2(20μg), A549 (lane 3(20μg), MDA-MB-231 (lane 4(20μg), HUVEC (lane 5(20μg) using Neuropilin-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Neuropilin-1 Antibody (YA3507) Product Image WB