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PHD2 Antibody (YA2431)

(Synonyms: EGLN1; C1orf12; ECYT3; HIFPH2; HPH2; PHD2; SM-20; SM20; ZMYND6; PHD2)
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Based on 1 Customer Validation

PHD2 Antibody (YA2431) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PHD2 Antibody (YA2431)
  • PHD2 Antibody (YA2431) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50

Product Details

Description

PHD2 Antibody (YA2431) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database

Entrez Gene: 54583 Human

SwissProt: Q9GZT9 Human

Immunogen

A synthesized peptide derived from human PHD2 / prolyl hydroxylase aa1-51/426.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PHD2 Antibody (YA2431)
    Western blot analysis of extracts from HEK293T (lane 2(20μg), HepG2 (lane 3(20μg), SH-SY5Y (lane 4(20μg), HCT116 (lane 5(20μg) using PHD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PHD2 is a HIF prolyl hydroxylase that regulates HIF-1 protein levels in mammalian cells. HIF-1α prolyl hydroxylases mainly include PHD1, PHD2, and PHD3. These three enzymes have sequence-similar catalytic domains and conserve amino acid residues for coordinating Fe (II) ions. Under hypoxic conditions, due to insufficient oxygen supply, the hydroxylase activity of PHDs decreases, leading to HIF-1α accumulation and inducing the expression of HRE-regulated target genes. Therefore, PHDs are considered intracellular oxygen sensors. PHD1 is found only in the cell nucleus, while PHD2 is mainly located in the cytoplasm.

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of human substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in human heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in human heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle

  • Isoforms & Post-Translational Modification

    Q9GZT9 has 3 isomers: Q9GZT9-1: 46021 Da (predicted); Q9GZT9-2: 43666 Da (predicted); Q9GZT9-3: 36486 Da (predicted).
    S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-323 or Cys-326

  • Subunit

    Monomer. Interacts with ING4; the interaction inhibits the hydroxylation of HIF alpha proteins. Interacts with PTGES3 (via PXLE motif); thereby recruiting EGLN1 to the HSP90 pathway to facilitate HIF alpha proteins hydroxylation. Interacts with LIMD1. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Interacts with EPAS1. Interacts with CBFA2T3 (PubMed:25974097). Interacts with HIF1A (PubMed:25974097)

  • SwissProt ID

    Q9GZT9

  • Gene ID
    54583 [NCBI]
  • Synonyms

    EGLN1; C1orf12; ECYT3; HIFPH2; HPH2; PHD2; SM-20; SM20; ZMYND6; PHD2

  • Research Field

    Cardiovascular

[1]. Yasumoto K, et al. Role of the intracellular localization of HIF-prolyl hydroxylases. Biochim Biophys Acta. 2009 May;1793(5):792-7. [Content Brief]

PHD2 Antibody (YA2431) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg), HepG2 (lane 3(20μg), SH-SY5Y (lane 4(20μg), HCT116 (lane 5(20μg) using PHD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PHD2 Antibody (YA2431) Product Image
    1/16
  • Experimental Validation Results for PHD2 Antibody (YA2431)
  • PHD2 Antibody (YA2431) Product Image
  • Experimental Validation Results for PHD2 Antibody (YA2431)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg), HepG2 (lane 3(20μg), SH-SY5Y (lane 4(20μg), HCT116 (lane 5(20μg) using PHD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHD2 Antibody (YA2431)
  • PHD2 Antibody (YA2431) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane 2(20μg), HepG2 (lane 3(20μg), SH-SY5Y (lane 4(20μg), HCT116 (lane 5(20μg) using PHD2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PHD2 Antibody (YA2431) Product Image WB