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HPDL Antibody (YA3042)

(Synonyms: 4-hydroxyphenylpyruvate dioxygenase like; GLOXD1; 4-HPPD-L)
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Based on 1 Customer Validation

HPDL Antibody (YA3042) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HPDL.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HPDL Antibody (YA3042)
  • HPDL Antibody (YA3042) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

HPDL Antibody (YA3042) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HPDL.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database

Entrez Gene: 84842 Human

SwissProt: Q96IR7 Human

Immunogen

A synthetic peptide of human HPDL

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105410

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HPDL Antibody (YA3042)
    Western blot analysis of extracts from MCF-7(lane 2(20ug) , Hela(lane 3(20ug) and A549(lane 4(20ug) using HPDL Antibody(HY-P83297) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    HPDL is an Iron-dependent dioxygenase that catalyzes the conversion of 4-hydroxyphenylpyruvate (4-HPPA) to 4-hydroxymandelate (4-HMA) in the mitochondria, one of the steps in the biosynthesis of coenzyme Q10 from tyrosine

  • Subcellular Localization

    Mitochondrion

  • SwissProt ID

    Q96IR7

  • Gene ID
    84842 [NCBI]
  • Synonyms

    4-hydroxyphenylpyruvate dioxygenase like; GLOXD1; 4-HPPD-L

  • Research Field

    Signal Transduction

HPDL Antibody (YA3042) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , Hela(lane 3(20ug) and A549(lane 4(20ug) using HPDL Antibody(HY-P83297) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HPDL Antibody (YA3042) Product Image
    1/16
  • Experimental Validation Results for HPDL Antibody (YA3042)
  • HPDL Antibody (YA3042) Product Image
  • Experimental Validation Results for HPDL Antibody (YA3042)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , Hela(lane 3(20ug) and A549(lane 4(20ug) using HPDL Antibody(HY-P83297) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HPDL Antibody (YA3042)
  • HPDL Antibody (YA3042) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug) , Hela(lane 3(20ug) and A549(lane 4(20ug) using HPDL Antibody(HY-P83297) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HPDL Antibody (YA3042) Product Image WB