PHAPI2/APRIL Antibody (YA2258)

Cat. No.: HY-P82513: Data Sheet COA
SDS
User Guide for Antibodies
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PHAPI2/APRIL Antibody (YA2258) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHAPI2/APRIL.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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PHAPI2/APRIL Antibody (YA2258) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

PHAPI2/APRIL Antibody (YA2258) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHAPI2/APRIL.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 27 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O75888

Gene ID

10541 [NCBI]

Immunogen

A synthesized peptide derived from human APRIL aa170-250/250.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PHAPI2/APRIL Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A549 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages

Induction:Down-regulated by phorbol myristate acetate/ionomycin treatment

Isoforms & Post-Translational Modification：O75888 has 6 isomers: O75888-1: 27433 Da (predicted); O75888-2: 25678 Da (predicted); O75888-3: 27059 Da (predicted); O75888-4: 24151 Da (predicted); O43508-2: 36589 Da (predicted); O75888-5: 22488 Da (predicted).
The precursor is cleaved by furin

Subunit：Homotrimer

RRID

AB_3104619

Database

Entrez Gene: 10541 Human ; 67628 Mouse ; 170724 Rat

SwissProt: Q92688 Human ; Q9EST5 Mouse ; Q9EST6 Rat

OMIM: 619823 Human

Research Field

Immunology

Synonyms

APRIL; CD256; TALL2; TNFSF13; TRDL1; ZTNF2

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PHAPI2/APRIL Antibody (YA2258) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PHAPI2/APRIL Antibody (YA2258)

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