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PHAPI2/APRIL Antibody (YA2258)

(Synonyms: APRIL; CD256; TALL2; TNFSF13; TRDL1; ZTNF2)
Customer Review

Based on 1 Customer Validation

PHAPI2/APRIL Antibody (YA2258) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHAPI2/APRIL.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • PHAPI2/APRIL Antibody (YA2258) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

PHAPI2/APRIL Antibody (YA2258) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHAPI2/APRIL.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human APRIL aa170-250/250.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104619

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PHAPI2/APRIL Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
    Immunocytochemistry analysis of RAW264.7 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
    Immunocytochemistry analysis of A549 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PHAPI2/APRIL is a Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages

    Induction:Down-regulated by phorbol myristate acetate/ionomycin treatment

  • Isoforms & Post-Translational Modification

    O75888 has 6 isomers: O75888-1: 27433 Da (predicted); O75888-2: 25678 Da (predicted); O75888-3: 27059 Da (predicted); O75888-4: 24151 Da (predicted); O43508-2: 36589 Da (predicted); O75888-5: 22488 Da (predicted).
    The precursor is cleaved by furin

  • Subunit

    Homotrimer

  • SwissProt ID

    O75888

  • Gene ID
    10541 [NCBI]
  • Synonyms

    APRIL; CD256; TALL2; TNFSF13; TRDL1; ZTNF2

  • Research Field

    Immunology

PHAPI2/APRIL Antibody (YA2258) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PHAPI2/APRIL Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of RAW264.7 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHAPI2/APRIL Antibody (YA2258) Product Image
    1/16
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • PHAPI2/APRIL Antibody (YA2258) Product Image
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PHAPI2/APRIL Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of RAW264.7 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • Experimental Validation Results for PHAPI2/APRIL Antibody (YA2258)
  • PHAPI2/APRIL Antibody (YA2258) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using PHAPI2/APRIL Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PHAPI2/APRIL Antibody (YA2258) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of RAW264.7 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHAPI2/APRIL Antibody (YA2258) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling PHAPI2 with PHAPI2 Antibody (HY-P82513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHAPI2 Antibody (HY-P82513) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHAPI2/APRIL Antibody (YA2258) Product Image WB
  • PHAPI2/APRIL Antibody (YA2258) Product Image ICC
  • PHAPI2/APRIL Antibody (YA2258) Product Image ICC