Hsp70 Antibody (YA728)

Cat. No.: HY-P80412: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Hsp70 Antibody (YA728) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hsp70.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Hsp70 Antibody (YA728) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Hsp70 Antibody (YA728) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hsp70.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 70 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P34931

Gene ID

15511 [NCBI]

Immunogen

Synthetic Peptide of HSP70

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-300

Sensitivity	Endogenous	Purity	Immunogen affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in 1*PBS ( pH 7.4) and 50% Glycerol. Preservative: 0.02% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB ICC

Western blot analysis of extracts from A431 (lane2(20μg), HEK293 (lane3(20μg), Hela (lane4(20μg) and Jurkat (lane5(20μg) using Hsp70 Antibody (HY-P80412). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling HSP70 Antibody (HY-P80412) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSP70 Antibody (HY-P80412) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH/3T3 cells labeling HSP70 Antibody (HY-P80412) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSP70 Antibody (HY-P80412) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365). Positive regulator of PRKN translocation to damaged mitochondria (PubMed:24270810)

Expression：
Tissue_specificity:Expression in sperm cells

Induction:Not induced by heat shock

Subunit：Interacts with PRKN

RRID

AB_3102496

Database

Entrez Gene: 15511 Human ; 3303 Human ; 193740 Mouse ; 3304 Mouse ; 24472 Rat 294254

SwissProt: P0DMV8 Human ; P0DMV9 Human ; P17879 Mouse ; Q61696 Mouse ; P0DMW0 Rat P0DMW1

OMIM: 140550 Human

Research Field

Signal Transduction

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Hsp70 Antibody (YA728) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Hsp70 Antibody (YA728)

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