Nova1 Antibody (YA1421)

製品番号: HY-P81676: データシート SDS COA User Guide for Antibodies
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Nova1 Antibody (YA1421) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nova1.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

Nova1 Antibody (YA1421) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nova1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 52 kDa;

Observed band size: 52 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P51513

Gene ID

4857 [NCBI]

Immunogen

A synthetic peptide of human Nova1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:500

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed:10811881). Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed in cerebellum, brain stem, hippocampus, and frontal cortex

Isoforms & Post-Translational Modification：P51513 has 3 isomers: P51513-4: 51727 Da (predicted); P51513-2: 19491 Da (predicted); P51513-5: 49253 Da (predicted).

Subunit：Interacts with PTBP2; the interaction is direct

Database

Entrez Gene: 4857 Human ; 664883 Mouse ;

SwissProt: P51513 Human ; Q9JKN6 Mouse ; Q80WA4 Rat

OMIM: 602157 Human

Research Field

Neuroscience

Synonyms

Nova-1

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)