Glutaminase Antibody (YA6288)

Cat. No.: HY-P86596: Data Sheet User Guide for Antibodies
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Glutaminase Antibody (YA6288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutaminase.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Glutaminase Antibody (YA6288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutaminase.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 55-65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O94925

Gene ID

2744 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
WB WB: Western Blot	1:1000-5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-20000
IP IP: Immunoprecipitation	1:50-200

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30239721, PubMed:30575854, PubMed:30970188); Lacks catalytic activity

Subcellular Localization：Mitochondrion; Cytoplasm, cytosol; Mitochondrion; Mitochondrion matrix; Mitochondrion matrix

Expression：
Tissue_specificity:Isomers 1 and 3 were detected in the cerebral cortex. Isomer 3 was highly expressed in astrocytomas, gangliogliomas, and ependymomas. Isomer 1 was highly expressed in the brain and kidneys, but not detected in the liver. Isomer 3 was highly expressed in the heart and pancreas, and expressed at lower levels in the placenta, lungs, pancreas, and kidneys, but not detected in the liver. Isomer 2 was expressed in cardiac and skeletal muscle.

Isoforms & Post-Translational Modification：O94925 has 3 isomers: O94925-1: 73461 Da (predicted); O94925-2: 17738 Da (predicted); O94925-3: 65460 Da (predicted).
Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits

Subunit：Homotetramer, dimer of dimers (PubMed:22538822, PubMed:26988803, PubMed:28526749, PubMed:29317493). The tetramers can assemble into rod-like oligomers (in vitro), but the physiological significance of this is not clear (By similarity). Interacts with RAF1 and MAP2K2 (PubMed:22538822). Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity

Documentation

User Guide for Antibodies (1077 KB)