Glutaminase Antibody (YA6288)
(Synonyms: GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase)Glutaminase Antibody (YA6288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutaminase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, IF-Tissue
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Reactivity :
Human, Rat
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1,000-1:2,000 | 1:50-1:200 | 1:50-1:200 | 1:200-1:1,000 | 1:1,000 |
Product Details
Glutaminase Antibody (YA6288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutaminase.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Rat Predicted Reactivity: Mouse
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 55-65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 68 kDa
Synthetic peptide within human Glutaminase aa 49-100.
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Glutaminase is the initial enzyme in glutamine metabolism, which catalyzes the hydrolysis of glutamine to glutamate in cells. Glutaminase plays a key role in cancer cell metabolism, growth, and proliferation. In mammalian cells, there are two paralogous GLS genes, GLS1 (or GLS) and GLS2. GLS1 encodes two alternatively spliced isozymes: kidney glutaminase (KGA) and glutaminase C (GAC) . GLS2 also encodes two isozymes: liver glutaminase (LGA) and glutaminase B.
GLS1 is ubiquitously expressed in various tissues, and its expression can be induced by the oncogene MYC. GLS1 is frequently activated and/or overexpressed in various types of cancer, including hepatocellular carcinoma (HCC) . GLS1 has been reported to promote tumorigenesis in different types of cancer, including HCC, which is mainly attributable to its glutaminase activity and role in promoting glutamine metabolism. GLS has emerged as a critical enzyme in a number of cancer types. Elevated GLS2 enzymatic activity has also been correlated with tumor cell growth in vitro and in vivo. N-Myc activates GLS2 to promote conversion of glutamine to glutamate in MYCN-amplified neuroblastoma cells. Abrogation of GLS2 function profoundly inhibits glutaminolysis and dramatically decreases cell proliferation and survival in vitro and in vivo. However, there is controversy over the role of GLS2 as a tumor suppressor. Enzymatic activity independent of GLS2 is up-regulated via p53 or p63 and plays a role of tumor suppressor.
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Subcellular Localization
Mitochondrion; Cytoplasm, cytosol; Mitochondrion; Mitochondrion matrix; Mitochondrion matrix
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Expression
Tissue_specificity:Isomers 1 and 3 were detected in the cerebral cortex. Isomer 3 was highly expressed in astrocytomas, gangliogliomas, and ependymomas. Isomer 1 was highly expressed in the brain and kidneys, but not detected in the liver. Isomer 3 was highly expressed in the heart and pancreas, and expressed at lower levels in the placenta, lungs, pancreas, and kidneys, but not detected in the liver. Isomer 2 was expressed in cardiac and skeletal muscle. -
Isoforms & Post-Translational Modification
O94925 has 3 isomers: O94925-1: 73461 Da (predicted); O94925-2: 17738 Da (predicted); O94925-3: 65460 Da (predicted).
Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits -
Subunit
Homotetramer, dimer of dimers (PubMed:22538822, PubMed:26988803, PubMed:28526749, PubMed:29317493). The tetramers can assemble into rod-like oligomers (in vitro), but the physiological significance of this is not clear (By similarity). Interacts with RAF1 and MAP2K2 (PubMed:22538822). Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity
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SwissProt ID
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Synonyms
GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase
Documentation
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