SREBP-1 Antibody

Cat. No.: HY-P80900: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

SREBP-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SREBP-1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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SREBP-1 Antibody Featured Recommendations:

2 Publications Citing Use of MCE SREBP-1 Antibody

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

SREBP-1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SREBP-1.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 122 kDa;

Observed band size: 60-70,122 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P36956

Gene ID

6720 [NCBI]

Immunogen

Synthetic peptide corresponding to Human SREBP-1.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20μg) , HepG2(lane 3(20ug) and HEK293(lane 4(40ug) using SREBP1 Antibody (HY-P80900) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane (PubMed:32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis (By similarity); Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis (PubMed:12177166, PubMed:32322062, PubMed:8402897). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed:12177166, PubMed:8402897). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation (PubMed:12177166, PubMed:32322062, PubMed:8402897); Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C (By similarity). Able to stimulate both lipogenic and cholesterogenic gene expression (PubMed:12177166, PubMed:32497488). Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver (By similarity). Required for innate immune response in macrophages by regulating lipid metabolism (By similarity); Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A (By similarity). Primarily controls expression of lipogenic gene (PubMed:12177166). Strongly activates global lipid synthesis in rapidly growing cells (By similarity); The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters; The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein; Nucleus; Nucleus; Nucleus

Expression：
Tissue_specificity:This gene is expressed in various tissues, with the highest abundance in the liver and adrenal glands (PubMed:8402897) . In fetal tissues, the highest expression levels are found in the lungs and liver (PubMed:8402897) ; it is dominant in hepatocellular carcinoma cell lines (PubMed:8402897) . This gene is also expressed in the kidneys, brain, white adipose tissue, and muscle (PubMed:8402897) ; it is primarily expressed in the liver and adipose tissue (PubMed:8402897) . This gene is also expressed in the kidneys, brain, white adipose tissue, and muscle (PubMed:8402897) .

Isoforms & Post-Translational Modification：P36956 has 6 isomers: P36956-1: 121675 Da (predicted); P36956-2: 113562 Da (predicted); P36956-3: 111102 Da (predicted); P36956-4: 124635 Da (predicted); P36956-5: 48276 Da (predicted); P36956-6: 45816 Da (predicted).
Processed in the Golgi apparatus, releasing the protein from the membrane (PubMed:32322062, PubMed:8626610). At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum (PubMed:32322062, PubMed:8626610). In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease (PubMed:32322062, PubMed:8626610). The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane (PubMed:32322062);Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression (By similarity). Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity);SCAP-free SREBF1 is ubiquitinated by the BCR(ARMC5) complex, leading to its degradation;Ubiquitinated; the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus

Subunit：Forms a tight complex with SCAP, the SCAP-SREBP complex, in the endoplasmic reticulum membrane and the Golgi apparatus (PubMed:32322062). Interacts with PAQR3; the interaction anchors the SCAP-SREBP complex to the Golgi apparatus in low cholesterol conditions (PubMed:26311497)

RRID

AB_3102744

Database

Entrez Gene: 6720 Human ; 20787 Mouse ; 78968 Rat

SwissProt: P36956 Human ; Q9WTN3 Mouse ; P56720 Rat

OMIM: 619016 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

SREBP-1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SREBP-1 Antibody

SREBP-1 Antibody Featured Recommendations:

2 Publications Citing Use of MCE SREBP-1 Antibody

Product Detail

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Background

Documentation

SREBP-1 Antibody Related Classifications

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