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Glutamine Synthetase Antibody (YA751)

(Synonyms: GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL)
Customer Review

Based on 1 Customer Validation

Glutamine Synthetase Antibody (YA751) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Glutamine Synthetase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
  • Glutamine Synthetase Antibody (YA751) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:100

Product Details

Description

Glutamine Synthetase Antibody (YA751) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Glutamine Synthetase.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Glutamine synthetase.AA range:190-373.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102492

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HeLa (lane 3), and 293T (lane 4) using Glutamine Synthetase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
    Western blot analysis of extracts from MCF-7(lane 2(20μg), K562(lane 3(20μg), Jurkat(lane 4(20μg) and HEK293(lane 5(20μg) ) using Glutamine Synthetase (HY-P80408) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80408, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Glutamine Synthetase is a Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia. Plays a key role in ammonium detoxification during erythropoiesis: the glutamine synthetase activity is required to remove ammonium generated by porphobilinogen deaminase (HMBS) during heme biosynthesis to prevent ammonium accumulation and oxidative stress. Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis. Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytosol; Microsome; Mitochondrion; Cell membrane; Lipid-anchor

  • Expression


    Tissue_specificity:Expression in endothelial cells

    Induction:By glucocorticoids. Vitamin D and the Wnt signaling pathway inhibit its expression and activity (PubMed:18555765) . Glutamine synthase (GS) levels are regulated by a negative feedback mechanism where elevated glutamine levels stimulate GS ubiquitin-mediated proteasomal degradation (PubMed:26990986, PubMed:38579670)

  • Subunit

    Decamer; composed of two pentamers (PubMed:18005987). Interacts with PALMD (By similarity). Interacts with RHOJ (PubMed:30158707). Interacts with BEST2; this interaction tethers a fraction of GLUL to the membrane, causing a decrease of cytosolic glutamine synthase (GS) activity and inhibits the chloride channel activity of BEST2 by affecting the gating at the aperture in the absence of intracellular glutamate (PubMed:36289327)

  • SwissProt ID

    P15104

  • Gene ID
    2752 [NCBI]
  • Synonyms

    GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL

  • Research Field

    Neuroscience

[1]. Häberle J, et al. Congenital glutamine deficiency with glutamine synthetase mutations. N Engl J Med. 2005 Nov 3;353(18):1926-33. [Content Brief]

[2]. Eelen G, et al. Role of glutamine synthetase in angiogenesis beyond glutamine synthesis. Nature. 2018 Sep;561(7721):63-69. [Content Brief]

[3]. Owji AP, et al. Bestrophin-2 and glutamine synthetase form a complex for glutamate release. Nature. 2022 Nov;611(7934):180-187. [Content Brief]

[4]. Vermeulen T, et al. Glutamine synthetase is essential for proliferation of fetal skin fibroblasts. Arch Biochem Biophys. 2008 Oct 1;478(1):96-102. [Content Brief]

[5]. Badertscher L, et al. Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells. Cell Rep. 2015 Dec 29;13(12):2879-91. [Content Brief]

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Glutamine Synthetase Antibody (YA751) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HeLa (lane 3), and 293T (lane 4) using Glutamine Synthetase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg), K562(lane 3(20μg), Jurkat(lane 4(20μg) and HEK293(lane 5(20μg) ) using Glutamine Synthetase (HY-P80408) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80408, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Glutamine Synthetase Antibody (YA751) Product Image
    1/16
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
  • Glutamine Synthetase Antibody (YA751) Product Image
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HeLa (lane 3), and 293T (lane 4) using Glutamine Synthetase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg), K562(lane 3(20μg), Jurkat(lane 4(20μg) and HEK293(lane 5(20μg) ) using Glutamine Synthetase (HY-P80408) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80408, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
  • Experimental Validation Results for Glutamine Synthetase Antibody (YA751)
  • Glutamine Synthetase Antibody (YA751) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 2), HeLa (lane 3), and 293T (lane 4) using Glutamine Synthetase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Glutamine Synthetase Antibody (YA751) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20μg), K562(lane 3(20μg), Jurkat(lane 4(20μg) and HEK293(lane 5(20μg) ) using Glutamine Synthetase (HY-P80408) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80408, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Glutamine Synthetase Antibody (YA751) Product Image WB
  • Glutamine Synthetase Antibody (YA751) Product Image WB