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Glucose Transporter GLUT3 Antibody (YA2922)(PBS only)

(Synonyms: SLC2A3; GLUT3; Solute carrier family 2; facilitated glucose transporter member 3; Glucose transporter type 3; brain; GLUT-3)

Glucose Transporter GLUT3 Antibody (YA2922) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose Transporter GLUT3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glucose Transporter GLUT3 Antibody (YA2922)(PBS only)
  • Glucose Transporter GLUT3 Antibody (YA2922)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Glucose Transporter GLUT3 Antibody (YA2922) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucose Transporter GLUT3.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Immunogen

A synthetic peptide of human Glucose Transporter GLUT3

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucose Transporter GLUT3 Antibody (YA2922)(PBS only)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), RAW264.7 (lane 3(20μg) and C6 (lane 4(20μg) using Glucose Transporter GLUT3 (HY-P83177) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Glucose Transporter GLUT3 is a Facilitative glucose transporter. Can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport. Required for mesendoderm differentiation[1][2][3].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Perikaryon; Cell projection

  • Expression


    Tissue_specificity:Highly expressed in brain (PubMed:8457197) . Expressed in many tissues

  • Subunit

    Interacts with SMIM43; the interaction may promote SLC2A3-mediated glucose transport to meet the energy needs of mesendoderm differentiation

  • SwissProt ID

    P11169

  • Gene ID
    144195 [NCBI]
  • Synonyms

    SLC2A3; GLUT3; Solute carrier family 2; facilitated glucose transporter member 3; Glucose transporter type 3; brain; GLUT-3

  • Research Field

    Signal Transduction

Glucose Transporter GLUT3 Antibody (YA2922)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), RAW264.7 (lane 3(20μg) and C6 (lane 4(20μg) using Glucose Transporter GLUT3 (HY-P83177) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Glucose Transporter GLUT3 Antibody (YA2922)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Glucose Transporter GLUT3 Antibody (YA2922)(PBS only)
  • Glucose Transporter GLUT3 Antibody (YA2922)(PBS only) Product Image
  • Experimental Validation Results for Glucose Transporter GLUT3 Antibody (YA2922)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), RAW264.7 (lane 3(20μg) and C6 (lane 4(20μg) using Glucose Transporter GLUT3 (HY-P83177) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucose Transporter GLUT3 Antibody (YA2922)(PBS only)
  • Glucose Transporter GLUT3 Antibody (YA2922)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), RAW264.7 (lane 3(20μg) and C6 (lane 4(20μg) using Glucose Transporter GLUT3 (HY-P83177) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Glucose Transporter GLUT3 Antibody (YA2922)(PBS only) Product Image WB