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Bax Antibody (YA826)

(Synonyms: Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4)
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Based on 1 Customer Validation

Bax Antibody (YA826) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Bax.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Monkey

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for Bax Antibody (YA826)
  • Experimental Validation Results for Bax Antibody (YA826)
  • Experimental Validation Results for Bax Antibody (YA826)
  • Bax Antibody (YA826) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Bax Antibody (YA826) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Bax.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Monkey
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Bax.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102613

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Bax Antibody (YA826)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80564, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA826)
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA826)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80564) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    BAX (BCL2-associated X protein) is a pro-apoptotic member of the BCL-2 family that regulates programmed cell death through mitochondrial apoptosis and functions as a critical determinant of cellular survival following apoptotic stimuli[1][2]. BAX forms heterodimers with anti-apoptotic BCL-2 proteins, and the balance between BAX and BCL-2 influences mitochondrial outer membrane permeabilization (MOMP), cytochrome c release, and activation of the intrinsic apoptotic pathway[2][3][4]. Mechanistically, BAX belongs to the pore-forming executioner subgroup of the BCL-2 family and undergoes activation in response to cellular stress signals, including p53-dependent apoptotic signaling, thereby promoting mitochondrial membrane permeabilization and irreversible cell death commitment[1][3][5]. In disease models, dysregulated BAX activity contributes to the pathophysiology of cancer, neurodegenerative disorders, and other apoptosis-associated conditions, making mitochondrial apoptosis a major target for therapeutic intervention[3][4][6]. Compared with related BCL-2 family isoforms, BAX is distinguished by its direct executioner function in apoptosis, whereas many alternative BCL-2 family isoforms display variable anti-apoptotic or context-dependent activities that remain incompletely characterized[3]. A notable BAX isoform, BaxΔ2, retains pro-apoptotic activity despite N-terminal alterations, highlighting functional diversity within the BAX locus and providing experimental models for studying non-canonical apoptotic mechanisms[7]. For experimental applications, BAX-dependent signaling is widely used to evaluate mitochondrial apoptosis, while therapeutic strategies targeting BCL-2 family interactions, including BH3 mimetics, are actively investigated to modulate BAX-mediated cell death in cancer research[3][6].

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass membrane protein; Cytoplasm; Nucleus; Cytoplasm; Cytoplasm; Cytoplasm

  • Expression


    Tissue_specificity:This gene is expressed in a variety of tissues. The Psi isoform is present in gliomas. The α isoform is expressed in the spleen, breast, ovary, testis, colon, and brain, and at low levels in the skin and lungs. The Sigma isoform is expressed in the spleen, breast, ovary, testis, lung, colon, and brain, and at low levels in the skin. Both the α and Sigma isoforms are expressed in promyelocytic leukemia, histiocytic lymphoma, Burkitt lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovarian adenocarcinoma, prostate cancer, prostate adenocarcinoma, lung cancer, epidermoid carcinoma, small cell lung cancer, and colon adenocarcinoma cell lines.

  • Isoforms & Post-Translational Modification

    Q07812 has 8 isomers: Q07812-1: 21184 Da (predicted); Q07812-2: 24220 Da (predicted); Q07812-3: 4678 Da (predicted); Q07812-4: 15772 Da (predicted); Q07812-5: 18129 Da (predicted); Q07812-6: 12887 Da (predicted); Q07812-7: 19312 Da (predicted); Q07812-8: 19718 Da (predicted).
    Ubiquitinated in the absence of XRCC6/Ku70 (PubMed:18362350). Ubiquitination promotes protein degradation (PubMed:18362350). Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12

  • Subunit

    Homodimer. Forms higher oligomers under stress conditions. Forms heterooligomers with BAK (PubMed:29531808). Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1 (PubMed:25609812). Interacts with SH3GLB1. Interacts with humanin; forms fibers with humanin which results in BAX conformational changes and sequestering of BAX into the fibers, preventing BAX activation (PubMed:12732850, PubMed:31690630). Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with RTL10/BOP. Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and consequently cytochrome c release from mitochondria. Interacts with GIMAP3/IAN4 and GIMAP5/IAN5; this interaction is increased, when cells initiate apoptosis upon IL2 withdrawal (PubMed:16509771). Interacts with IRF3; the interaction is direct, increases upon Sendai virus infection and mediates the formation of the apoptosis complex TOMM70:HSP90AA1:IRF3:BAX (PubMed:25609812). Interacts with MOAP1, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Interacts with BCL2L10/BCL-B (PubMed:23235460). Interacts with non-acetylated XRCC6/Ku70; this interaction leads to BAX sequestration in the cytosol, away from the mitochondria, preventing BAX-mediated apoptosis (PubMed:15023334)

  • SwissProt ID

    Q07812

  • Gene ID
    581 [NCBI]
  • Synonyms

    Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4

  • Research Field

    Cell Biology

[1]. Brady HJ, et al. Bax. The pro-apoptotic Bcl-2 family member, Bax. Int J Biochem Cell Biol. 1998 Jun;30(6):647-50. [Content Brief]

[2]. Bax gene information from NCBI.

[3]. Warren CFA, et al. BCL-2 family isoforms in apoptosis and cancer. Cell Death Dis. 2019 Feb 21;10(3):177. [Content Brief]

[4]. Vogler M, et al. The BCL2 family: from apoptosis mechanisms to new advances in targeted therapy. Signal Transduct Target Ther. 2025 Mar 21;10(1):91. [Content Brief]

[5]. Maes ME, et al. BAX to basics: How the BCL2 gene family controls the death of retinal ganglion cells. Prog Retin Eye Res. 2017 Mar;57:1-25. [Content Brief]

[6]. Huang Y, et al. The adjuvant treatment role of ω-3 fatty acids by regulating gut microbiota positively in the acne vulgaris. J Dermatolog Treat. 2024 Dec;35(1):2299107. [Content Brief]

[7]. Rabezanahary H, et al. Live virus neutralizing antibodies against pre and post Omicron strains in food and retail workers in Québec, Canada. Heliyon. 2024 May 21;10(10):e31026. [Content Brief]

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Bax Antibody (YA826) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80564, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80564) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA826) Product Image
    1/16
  • Experimental Validation Results for Bax Antibody (YA826)
  • Experimental Validation Results for Bax Antibody (YA826)
  • Experimental Validation Results for Bax Antibody (YA826)
  • Bax Antibody (YA826) Product Image
  • Experimental Validation Results for Bax Antibody (YA826)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80564, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA826)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA826)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80564) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA826)
  • Experimental Validation Results for Bax Antibody (YA826)
  • Experimental Validation Results for Bax Antibody (YA826)
  • Bax Antibody (YA826) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80564, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA826) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA826) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80564) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA826) Product Image WB
  • Bax Antibody (YA826) Product Image WB
  • Bax Antibody (YA826) Product Image WB