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ZYX Antibody (YA2459)(PBS only)

(Synonyms: ESP2; HED2; ZYX; Zyxin2)

ZYX Antibody (YA2459) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZYX.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ZYX Antibody (YA2459)(PBS only)
  • ZYX Antibody (YA2459)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

ZYX Antibody (YA2459) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZYX.

  • Host Rabbit
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Immunogen

A synthetic peptide of human Zyxin

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ZYX Antibody (YA2459)(PBS only)
    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), 3T3 (lane 2, 15 μg), and C6 (lane 3, 15 μg) using ZYX Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ZYX is an Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton; Nucleus; Cell junction, focal adhesion

  • Isoforms & Post-Translational Modification

    Q15942 has 2 isomers: Q15942-1: 61277 Da (predicted); Q15942-2: 45075 Da (predicted).

  • Subunit

    Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Interacts with SYNPO2

  • SwissProt ID

    Q15942

  • Gene ID
    7791 [NCBI]
  • Synonyms

    ESP2; HED2; ZYX; Zyxin2

  • Research Field

    Neuroscience

ZYX Antibody (YA2459)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), 3T3 (lane 2, 15 μg), and C6 (lane 3, 15 μg) using ZYX Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • ZYX Antibody (YA2459)(PBS only) Product Image
    1/16
  • Experimental Validation Results for ZYX Antibody (YA2459)(PBS only)
  • ZYX Antibody (YA2459)(PBS only) Product Image
  • Experimental Validation Results for ZYX Antibody (YA2459)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), 3T3 (lane 2, 15 μg), and C6 (lane 3, 15 μg) using ZYX Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ZYX Antibody (YA2459)(PBS only)
  • ZYX Antibody (YA2459)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), 3T3 (lane 2, 15 μg), and C6 (lane 3, 15 μg) using ZYX Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • ZYX Antibody (YA2459)(PBS only) Product Image WB