P2RX7 Antibody (YA4449)(PBS only)

Cat. No.: HY-P84752A: Data Sheet User Guide for Antibodies
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P2RX7 Antibody (YA4449) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RX7.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

P2RX7 Antibody (YA4449) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RX7.

Host

Mouse

분자량

Predicted band size: 69 kDa;

Observed band size: 70-80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rabbit

SwissProt ID

Q99572

Gene ID

5027 [NCBI]

Immunogen

Purified recombinant fragment of human P2RX7 (AA: 226-452) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation (PubMed:17483156, PubMed:25281740, PubMed:9038151). Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux (PubMed:17483156, PubMed:20453110, PubMed:28235784, PubMed:39262850). Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules (PubMed:17483156). In microglia, may mediate NADPH transport across the plasma membrane (PubMed:39142135). In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1 (PubMed:26877061). Cooperates with KCNK6 to activate NLRP3 inflammasome (By similarity). Activates death pathways leading to apoptosis and autophagy (PubMed:21821797, PubMed:23303206, PubMed:28326637). Activates death pathways leading to pyroptosis (By similarity); Shows ion channel activity but no macropore function; Non-functional channel; Non-functional channel

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Widely expressed with highest levels in brain and immune tissues; Predominant form in many tissues

Isoforms & Post-Translational Modification：Q99572 has 9 isomers: Q99572-1: 68585 Da (predicted); Q99572-2: 41792 Da (predicted); Q99572-3: 14749 Da (predicted); Q99572-4: 49165 Da (predicted); Q99572-5: 31294 Da (predicted); Q99572-6: 35544 Da (predicted); Q99572-7: 31446 Da (predicted); Q99572-8: 58239 Da (predicted); Q99572-9: 29338 Da (predicted).
Phosphorylation results in its inactivation;ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel;Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface (PubMed:18971257). Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane (By similarity)

Subunit：Homotrimers (PubMed:20453110). Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB (PubMed:11707406). Interacts (via C-terminus) with EMP2 (PubMed:12107182). Interacts with isoform B; this interaction potentiates P2RX7 responses (PubMed:20453110)

Synonyms

P2X7

각종 서류

User Guide for Antibodies (1077 KB)