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Myelin Basic Protein Antibody(YA6611)

(Synonyms: GDB antibody; Golli MBP antibody; Golli MBP myelin basic protein antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_CAVPO antibody; MBP_HUMAN antibody; GDB antibody; Golli MBP antibody; Golli MBP myelin basic protein antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_CAVPO antibody; MBP_HUMAN antibody; MGC99675 antibody; MLD antibody; Myelin A1 protein antibody; Myelin A1 Protein, basic antibody; Myelin basic protein antibody; Myelin Deficient antibody; Myelin membrane encephalitogenic protein antibody; OTTHUMP00000163776 antibody; OTTHUMP00000174387 antibody; OTTHUMP00000174388 antibody; SHI antibody; Shiverer antibody; SP antibody; )
Customer Review

Based on 1 Customer Validation

Myelin Basic Protein Antibody(YA6611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue, mIHC

  • Reactivity :

    Human, Mouse, Rat, Monkey, Pig

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Myelin Basic Protein Antibody(YA6611) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:1000 1:1000-1:5000 1:500-1:1000 1:500-1:1000 1:2000

Product Details

Description

Myelin Basic Protein Antibody(YA6611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Pig
  • Observed Molecular Weight
    Observed band size: 14~25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719227

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
    Western blot analysis was performed on protein extracts from Mouse brain (lane 2, 20 μg), Mouse brain (lane 3, 40 μg), Rat brain (lane 4, 20 μg), and Rat brain (lane 5, 40 μg) using Myelin Basic Protein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Myelin Basic Protein is a The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation

  • Subcellular Localization

    Myelin membrane,Nucleus

  • Expression


    Tissue_Specificity: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system

  • Isoforms & Post-Translational Modification

    P02686 has 6 isomers: P02686-1: 33117 Da (predicted); P02686-2: 21522 Da (predicted); P02686-3: 21493 Da (predicted); P02686-4: 20246 Da (predicted); P02686-5: 18591 Da (predicted); P02686-6: 17343 Da (predicted).
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation丨The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6)丨Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated丨Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells丨Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1

  • Subunit

    Homodimer

  • SwissProt ID

    P02686

  • Gene ID
    4155 [NCBI]
  • Synonyms

    GDB antibody; Golli MBP antibody; Golli MBP myelin basic protein antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_CAVPO antibody; MBP_HUMAN antibody; GDB antibody; Golli MBP antibody; Golli MBP myelin basic protein antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_CAVPO antibody; MBP_HUMAN antibody; MGC99675 antibody; MLD antibody; Myelin A1 protein antibody; Myelin A1 Protein, basic antibody; Myelin basic protein antibody; Myelin Deficient antibody; Myelin membrane encephalitogenic protein antibody; OTTHUMP00000163776 antibody; OTTHUMP00000174387 antibody; OTTHUMP00000174388 antibody; SHI antibody; Shiverer antibody; SP antibody;

Myelin Basic Protein Antibody(YA6611) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Mouse brain (lane 2, 20 μg), Mouse brain (lane 3, 40 μg), Rat brain (lane 4, 20 μg), and Rat brain (lane 5, 40 μg) using Myelin Basic Protein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody(YA6611) Product Image
    1/16
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Myelin Basic Protein Antibody(YA6611) Product Image
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Mouse brain (lane 2, 20 μg), Mouse brain (lane 3, 40 μg), Rat brain (lane 4, 20 μg), and Rat brain (lane 5, 40 μg) using Myelin Basic Protein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Experimental Validation Results for Myelin Basic Protein Antibody(YA6611)
  • Myelin Basic Protein Antibody(YA6611) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts from Mouse brain (lane 2, 20 μg), Mouse brain (lane 3, 40 μg), Rat brain (lane 4, 20 μg), and Rat brain (lane 5, 40 μg) using Myelin Basic Protein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Myelin Basic Protein Antibody(YA6611) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody(YA6611) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myelin Basic Protein Antibody(YA6611) Product Image WB
  • Myelin Basic Protein Antibody(YA6611) Product Image IHC-P
  • Myelin Basic Protein Antibody(YA6611) Product Image IHC-P