Myelin Basic Protein Antibody(YA6611)

Cat. No.: HY-P86918: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Myelin Basic Protein Antibody(YA6611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Myelin Basic Protein Antibody(YA6611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myelin Basic Protein.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 14~25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey, Pig

SwissProt ID

P02686

Gene ID

4155 [NCBI]

Immunogen

Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:5000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:500-1:1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:500-1:1000
mIHC mIHC: Multiplex Immunohistochemical	1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis was performed on protein extracts from Mouse brain (lane 2, 20 μg), Mouse brain (lane 3, 40 μg), Rat brain (lane 4, 20 μg), and Rat brain (lane 5, 40 μg) using Myelin Basic Protein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Myelin Basic Protein Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation

Subcellular Localization：Myelin membrane,Nucleus

Expression：
Tissue_Specificity: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system

Isoforms & Post-Translational Modification：P02686 has 6 isomers: P02686-1: 33117 Da (predicted); P02686-2: 21522 Da (predicted); P02686-3: 21493 Da (predicted); P02686-4: 20246 Da (predicted); P02686-5: 18591 Da (predicted); P02686-6: 17343 Da (predicted).
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation丨The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6)丨Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated丨Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells丨Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1

Subunit：Homodimer

RRID

AB_3719227

Synonyms

GDB antibody; Golli MBP antibody; Golli MBP myelin basic protein antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_CAVPO antibody; MBP_HUMAN antibody; GDB antibody; Golli MBP antibody; Golli MBP myelin basic protein antibody; Hemopoietic MBP antibody; HMBPR antibody; HUGO antibody; MBP antibody; MBP_CAVPO antibody; MBP_HUMAN antibody; MGC99675 antibody; MLD antibody; Myelin A1 protein antibody; Myelin A1 Protein, basic antibody; Myelin basic protein antibody; Myelin Deficient antibody; Myelin membrane encephalitogenic protein antibody; OTTHUMP00000163776 antibody; OTTHUMP00000174387 antibody; OTTHUMP00000174388 antibody; SHI antibody; Shiverer antibody; SP antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)