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TIM 1 Antibody (YA3545)

(Synonyms: HAVCR; HAVCR 1; HA; Vcr-1; hepatitis A virus cellular receptor 1; HKIM-1; Kidney Injury Molecule1; KIM1; KIM 1; KIM-1; TIM-1; TIMD 1; TIMD1; KM-1; TIMD1_HUMAN; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell membrane protein 1. 肾脏损伤分子1)
Customer Review

Based on 1 Customer Validation

TIM 1 Antibody (YA3545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIM 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
  • TIM 1 Antibody (YA3545) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TIM 1 Antibody (YA3545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIM 1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human TIM 1.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103261

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TIM 1 Antibody (YA3545)
    Western blot analysis of extracts from HepG2(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) using TIM 1 Antibody (HY-P81186) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
    Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), U251MG (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), and PC-12 (lane 4, 15 μg) using TIM 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    TIM 1 is a Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions. As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses. Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease. Also regulates expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10. Acts as a regulator of T-cell proliferation. May play a role in kidney injury and repair; (Microbial infection) Acts as a receptor for Hepatitis A virus; (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane. Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP; (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane. TIM1 and Dengue virus are co-internalized during virus entry; (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E; (Microbial infection) Plays a positive role in Chikungunya virus cell entry[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is widely expressed, with the highest expression levels in the kidneys and testes. During the development of helper T cell responses, it is expressed by activated CD4+ T cells.

    Induction:Up-regulated in the kidney in renal diseases (at protein level)

  • Subunit

    Interacts with STAM (PubMed:29742433). Interacts with SELPLG (PubMed:24703780)

  • SwissProt ID

    Q96D42

  • Gene ID
    26762 [NCBI]
  • Synonyms

    HAVCR; HAVCR 1; HA; Vcr-1; hepatitis A virus cellular receptor 1; HKIM-1; Kidney Injury Molecule1; KIM1; KIM 1; KIM-1; TIM-1; TIMD 1; TIMD1; KM-1; TIMD1_HUMAN; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell membrane protein 1. 肾脏损伤分子1

TIM 1 Antibody (YA3545) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) using TIM 1 Antibody (HY-P81186) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), U251MG (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), and PC-12 (lane 4, 15 μg) using TIM 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TIM 1 Antibody (YA3545) Product Image
    1/16
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
  • TIM 1 Antibody (YA3545) Product Image
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) using TIM 1 Antibody (HY-P81186) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), U251MG (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), and PC-12 (lane 4, 15 μg) using TIM 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
  • Experimental Validation Results for TIM 1 Antibody (YA3545)
  • TIM 1 Antibody (YA3545) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) using TIM 1 Antibody (HY-P81186) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TIM 1 Antibody (YA3545) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), U251MG (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), and PC-12 (lane 4, 15 μg) using TIM 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TIM 1 Antibody (YA3545) Product Image WB
  • TIM 1 Antibody (YA3545) Product Image WB