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  5. TIM 1 Antibody (YA3545)

TIM 1 Antibody (YA3545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIM 1.

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사이즈 가격 재고 수량
20 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
or 대량구매 문의

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • 제품 설명

제품 설명

TIM 1 Antibody (YA3545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIM 1.
Host

Rabbit
Clonality

Monoclonal,Recombinant
분자량
Predicted band size: 39kDa;
Observed band size: 50kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human TIM 1.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HepG2(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) using TIM 1 Antibody (HY-P81186) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), U251MG (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), and PC-12 (lane 4, 15 μg) using TIM 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed:24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed:24703780). Also regulates expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (By similarity). Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (PubMed:17471468); (Microbial infection) Acts as a receptor for Hepatitis A virus; (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:21536871). Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP (PubMed:26487564); (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:23084921). TIM1 and Dengue virus are co-internalized during virus entry (PubMed:29742433); (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E; (Microbial infection) Plays a positive role in Chikungunya virus cell entry
Subcellular Localization：Cell membrane; Single-pass type I membrane protein
Expression：
Tissue_specificity:It is widely expressed, with the highest expression levels in the kidneys and testes. During the development of helper T cell responses, it is expressed by activated CD4+ T cells.

Induction:Up-regulated in the kidney in renal diseases (at protein level)
Subunit：Interacts with STAM (PubMed:29742433). Interacts with SELPLG (PubMed:24703780)
RRID
Database

Entrez Gene: 26762 Human ; 171283 Mouse ; 286934 Rat

SwissProt: Q96D42 Human ; Q5QNS5 Mouse ; O54947 Rat

OMIM: 606518 Human

Synonyms
HAVCR; HAVCR 1; HA; Vcr-1; hepatitis A virus cellular receptor 1; HKIM-1; Kidney Injury Molecule1; KIM1; KIM 1; KIM-1; TIM-1; TIMD 1; TIMD1; KM-1; TIMD1_HUMAN; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell membrane protein 1. 肾脏损伤分子1
각종 서류
SDS (367 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

TIM 1 Antibody (YA3545) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
TIM 1 Antibody (YA3545)
Cat. No.:
HY-P81186
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