TIM 1 Antibody (YA3545)

Cat. No.: HY-P81186: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TIM 1 Antibody (YA3545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIM 1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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TIM 1 Antibody (YA3545) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

TIM 1 Antibody (YA3545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIM 1.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 39kDa;

Observed band size: 50kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96D42

Gene ID

26762 [NCBI]

Immunogen

A synthetic peptide of human TIM 1.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2(lane 2(20ug) , Jurkat(lane 3(20ug) and NIH/3T3(lane 4(20ug) using TIM 1 Antibody (HY-P81186) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), U251MG (lane 2, 15 μg), RAW264.7 (lane 3, 15 μg), and PC-12 (lane 4, 15 μg) using TIM 1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed:24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed:24703780). Also regulates expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (By similarity). Acts as a regulator of T-cell proliferation (By similarity). May play a role in kidney injury and repair (PubMed:17471468); (Microbial infection) Acts as a receptor for Hepatitis A virus; (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:21536871). Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP (PubMed:26487564); (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:23084921). TIM1 and Dengue virus are co-internalized during virus entry (PubMed:29742433); (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E; (Microbial infection) Plays a positive role in Chikungunya virus cell entry

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is widely expressed, with the highest expression levels in the kidneys and testes. During the development of helper T cell responses, it is expressed by activated CD4+ T cells.

Induction:Up-regulated in the kidney in renal diseases (at protein level)

Subunit：Interacts with STAM (PubMed:29742433). Interacts with SELPLG (PubMed:24703780)

RRID

AB_3103261

Database

Entrez Gene: 26762 Human ; 171283 Mouse ; 286934 Rat

SwissProt: Q96D42 Human ; Q5QNS5 Mouse ; O54947 Rat

OMIM: 606518 Human

Synonyms

HAVCR; HAVCR 1; HA; Vcr-1; hepatitis A virus cellular receptor 1; HKIM-1; Kidney Injury Molecule1; KIM1; KIM 1; KIM-1; TIM-1; TIMD 1; TIMD1; KM-1; TIMD1_HUMAN; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell membrane protein 1. 肾脏损伤分子1

Documentation

Select Batch:

Data Sheet (232 KB) SDS (368 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

TIM 1 Antibody (YA3545) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TIM 1 Antibody (YA3545)

TIM 1 Antibody (YA3545) Featured Recommendations:

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Background

Documentation

TIM 1 Antibody (YA3545) Related Classifications

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