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ATP1B3 Antibody (YA2201)

(Synonyms: atp1b3; ATPB3; CD298; FLJ29027; NKAB3S; Sodium pump subunit beta 3; Sodium/potassium dependent ATPase subunit beta 3)
Customer Review

Based on 1 Customer Validation

ATP1B3 Antibody (YA2201) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1B3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • ATP1B3 Antibody (YA2201) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:5000 1:50-1:100

Product Details

Description

ATP1B3 Antibody (YA2201) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1B3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ATP1B3

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), A549 (lane 4), and Caco-2 (lane 5) using ATP1B3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Jurkat (lane 4, 20ug) and THP-1 (lane 5, 20ug) using ATP1B3 Antibody (HY-P82456). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Immunohistochemical analysis of paraffin-embedded human ‌‌liver cancer‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Immunohistochemical analysis of paraffin-embedded human ‌Stromal tumor‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Immunohistochemical analysis of paraffin-embedded human ‌spleen tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    ATP1B3 is a This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known

  • Subcellular Localization

    Apical cell membrane; Single-pass type II membrane protein; Basolateral cell membrane; Single-pass type II membrane protein; Melanosome

  • Isoforms & Post-Translational Modification

    P54709 has 2 isomers: P54709-1: 31513 Da (predicted); P54709-2: 21713 Da (predicted).

  • Subunit

    The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with catalytic alpha subunit ATP12A

  • SwissProt ID

    P54709

  • Gene ID
    483 [NCBI]
  • Synonyms

    atp1b3; ATPB3; CD298; FLJ29027; NKAB3S; Sodium pump subunit beta 3; Sodium/potassium dependent ATPase subunit beta 3

  • Research Field

    Signal Transduction

ATP1B3 Antibody (YA2201) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), A549 (lane 4), and Caco-2 (lane 5) using ATP1B3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Jurkat (lane 4, 20ug) and THP-1 (lane 5, 20ug) using ATP1B3 Antibody (HY-P82456). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌‌liver cancer‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Stromal tumor‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌spleen tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image
    1/16
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • ATP1B3 Antibody (YA2201) Product Image
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), A549 (lane 4), and Caco-2 (lane 5) using ATP1B3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Jurkat (lane 4, 20ug) and THP-1 (lane 5, 20ug) using ATP1B3 Antibody (HY-P82456). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌‌liver cancer‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Stromal tumor‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌spleen tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • Experimental Validation Results for ATP1B3 Antibody (YA2201)
  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), A549 (lane 4), and Caco-2 (lane 5) using ATP1B3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Jurkat (lane 4, 20ug) and THP-1 (lane 5, 20ug) using ATP1B3 Antibody (HY-P82456). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌‌liver cancer‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Stromal tumor‌ tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌spleen tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌placenta tissue using ATP1B3 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82456, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • ATP1B3 Antibody (YA2201) Product Image WB
  • ATP1B3 Antibody (YA2201) Product Image WB
  • ATP1B3 Antibody (YA2201) Product Image IHC-P
  • ATP1B3 Antibody (YA2201) Product Image IHC-P
  • ATP1B3 Antibody (YA2201) Product Image IHC-P
  • ATP1B3 Antibody (YA2201) Product Image IHC-P
  • ATP1B3 Antibody (YA2201) Product Image IHC-P
  • ATP1B3 Antibody (YA2201) Product Image IHC-P