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PHOX2B Antibody (YA3028)

(Synonyms: NBLST2; NBPhox; PHOX 2B; PHOX2B; PMX 2B; PMX2B)
Customer Review

Based on 1 Customer Validation

PHOX2B Antibody (YA3028) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHOX2B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • PHOX2B Antibody (YA3028) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200

Product Details

Description

PHOX2B Antibody (YA3028) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHOX2B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PHOX2B aa260-314/314.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PHOX2B Antibody (YA3028)
    Western blot analysis of extracts from Hela(lane 2(20μg) , RAW264.7 (lane 3(20μg) ,HT-29(lane 4(20μg)and HepG2( lane 5(20μg) using SOD-1 Antibody（HY-P83283）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/600) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
    Immunocytochemistry analysis of Hela cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
    Immunocytochemistry analysis of SH-SY5Y cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PHOX2B is involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expressed in neuroblastoma, brain and adrenal gland

  • Subunit

    Interacts with TRIM11

  • SwissProt ID

    Q99453

  • Gene ID
    8929 [NCBI]
  • Synonyms

    NBLST2; NBPhox; PHOX 2B; PHOX2B; PMX 2B; PMX2B

  • Research Field

    Neuroscience

PHOX2B Antibody (YA3028) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , RAW264.7 (lane 3(20μg) ,HT-29(lane 4(20μg)and HepG2( lane 5(20μg) using SOD-1 Antibody（HY-P83283）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/600) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHOX2B Antibody (YA3028) Product Image
    1/16
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • PHOX2B Antibody (YA3028) Product Image
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , RAW264.7 (lane 3(20μg) ,HT-29(lane 4(20μg)and HepG2( lane 5(20μg) using SOD-1 Antibody（HY-P83283）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/600) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • Experimental Validation Results for PHOX2B Antibody (YA3028)
  • PHOX2B Antibody (YA3028) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) , RAW264.7 (lane 3(20μg) ,HT-29(lane 4(20μg)and HepG2( lane 5(20μg) using SOD-1 Antibody（HY-P83283）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/600) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • PHOX2B Antibody (YA3028) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHOX2B Antibody (YA3028) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling PHOX2B Antibody with PHOX2B Antibody (YA3028) (HY-P83283) at 1/50dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHOX2B Antibody (YA3028) ((HY-P83283) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHOX2B Antibody (YA3028) Product Image WB
  • PHOX2B Antibody (YA3028) Product Image ICC
  • PHOX2B Antibody (YA3028) Product Image ICC