CaMKI Antibody (YA7281)
(Synonyms: Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, CAMK1)CaMKI Antibody (YA7281) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKI.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 | 1:50-200 |
Product Details
CaMKI Antibody (YA7281) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKI.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 41 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 41 kDa
A synthesized peptide derived from human CAMKI
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CaMK I is a Ca2+/calmodulin-dependent kinase positioned downstream of CaMKK, linking intracellular calcium signals to cell-cycle control, neuronal polarity, osteoclast differentiation, and transporter trafficking[1][2][3][4]. Mechanistically, CaMKK activates CaMKI, CaMKIV, and AMPKα, and CaMKI activity supports Ca2+-dependent signaling programs in neuronal, metabolic, and pathophysiological pathways[5]. In hippocampal neurons, TRPC5-dependent localized calcium influx activated CaMKK and CaMKIγ, and CaMKIγ, but not other CaMKI isoforms, promoted axon formation[2]. Compared with related isoforms, CaMKI regulates G1, whereas CaMKII regulates G2/M and the metaphase-anaphase transition, limiting direct extrapolation from CaMKII inhibitor studies to CaMKI biology[1]. In osteoclast models, CaMKI and CaMKIIγ were present during RANKL-induced differentiation, and KN-93 or KN-62 inhibited osteoclastogenesis, cathepsin K expression, ERK phosphorylation, CREB activity, and bone resorption[3]. For experimental applications, STO-609-derived TIM-063 directly inhibited CaMKK and suppressed ionomycin-induced phosphorylation of CaMKI, CaMKIV, and AMPKα in cells, supporting pathway-level interrogation rather than CaMKI-selective inhibition[5].
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Subcellular Localization
Cytoplasm,Nucleus
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Expression
Tissue_Specificity: Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex -
Isoforms & Post-Translational Modification
Q14012: 370 amino acids, molecular weight 41337 Da.
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Subunit
Monomer
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SwissProt ID
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Synonyms
Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, CAMK1
Documentation
References
[1]. Skelding KA, et al. Controlling the cell cycle: the role of calcium/calmodulin-stimulated protein kinases I and II. Cell Cycle. 2011 Feb 15;10(4):631-9. [Content Brief]
[2]. Davare MA, et al. Transient receptor potential canonical 5 channels activate Ca2+/calmodulin kinase Igamma to promote axon formation in hippocampal neurons. J Neurosci. 2009 Aug 5;29(31):9794-808. [Content Brief]
[4]. Sung U, et al. Ca2+ dependent surface trafficking of norepinephrine transporters depends on threonine 30 and Ca2+ calmodulin kinases. J Chem Neuroanat. 2017 Oct;83-84:19-35. [Content Brief]
[5]. Ohtsuka S, et al. Development and Characterization of Novel Molecular Probes for Ca2+/Calmodulin-Dependent Protein Kinase Kinase, Derived from STO-609. Biochemistry. 2020 May 5;59(17):1701-1710. [Content Brief]
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