TORC2 Antibody (YA4325)

Cat. No.: HY-P84628: Data Sheet User Guide for Antibodies
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TORC2 Antibody (YA4325) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TORC2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

TORC2 Antibody (YA4325) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TORC2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 73 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

Q53ET0

Gene ID

200186 [NCBI]

Immunogen

Purified recombinant fragment of human CRTC2 aa 406-615.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is most abundantly expressed in the thymus. It is also expressed in both B cells and T cells. High expression is observed in HEK293T cells and insulinomas. High levels of expression are also found in the spleen, ovaries, muscles, and lungs, with the highest expression in muscles. Lower expression is observed in the brain, colon, heart, kidneys, prostate, small intestine, and stomach. Weak expression is observed in the liver and pancreas.

Subunit：Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 (PubMed:15454081). 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction (PubMed:15454081). Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1 (PubMed:15454081). Interacts with SIK2 (PubMed:15454081). Interacts with 14-3-3 proteins, YWHAB and YWHAG (PubMed:15454081, PubMed:18626018). Interacts (probably when phosphorylated at Ser-171) with YWHAE (PubMed:30611118). Interacts with calmodulin-dependent catalytic subunit PPP3CA/calcineurin A (PubMed:15454081, PubMed:30611118). Interaction with COP1 mediates nuclear export and degradation of CRTC2 (By similarity)

Synonyms

TORC2; TORC-2; CRTC2

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/bxpc-3-cell.html [Content Brief]