Human IgG2 Antibody (YA2196)
(Synonyms: IGHG2; immunoglobulin Gm2; immunoglobulin heavy constant gamma 2 (G2m marker))Human IgG2 Antibody (YA2196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Human IgG2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:200 |
Product Details
Human IgG2 Antibody (YA2196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Human IgG2.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 36 kDa
A synthesized peptide derived from human IgG2 aa90-130.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Human IgG2 is a Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen[1][2][3].
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Subcellular Localization
Secreted; Cell membrane; Single-pass membrane protein
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Isoforms & Post-Translational Modification
P01859 has 2 isomers: P01859-2: 43806 Da (predicted); P01859-1: 35901 Da (predicted).
Glycosylation on Asn-176 is required for interaction with Fc receptors and ability to activate the complement pathway;(Microbial infection) Deglycosylation on Asn-176 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway -
Subunit
Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked
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SwissProt ID
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Synonyms
IGHG2; immunoglobulin Gm2; immunoglobulin heavy constant gamma 2 (G2m marker)
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Research Field
Immunology
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