AHA1 Antibody (YA2023)(PBS only)

Cat. No.: HY-P82278A: Data Sheet User Guide for Antibodies
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AHA1 Antibody (YA2023) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AHA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

AHA1 Antibody (YA2023) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AHA1.

Host

Rabbit

Molecular Weight

Predicted band size: 38 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O95433

Gene ID

10598 [NCBI]

Immunogen

A synthetic peptide of human AHA1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IP IP: Immunoprecipitation	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155)

Subcellular Localization：Cytoplasm, cytosol; Endoplasmic reticulum

Expression：
Tissue_specificity:Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta

Induction:By heat shock and treatment with the HSP90 inhibitor 17-demethoxygeldanamycin (17AAG)

Isoforms & Post-Translational Modification：O95433 has 2 isomers: O95433-1: 38274 Da (predicted); O95433-2: 32340 Da (predicted).
Phosphorylation at Tyr-223 enhances binding to chaperone HSP90AA1

Subunit：Interacts with HSPCA/HSP90 (PubMed:12504007, PubMed:12604615). Interacts (phosphorylated on Tyr-223) with HSP90AA1; the interaction activates HSP90AA1 ATPase activity (PubMed:27353360, PubMed:29127155). Interacts with HSP90AB1 (By similarity). Interacts with GCH1 (PubMed:16696853). Interacts with SRPK1 (PubMed:19240134). Interacts with FLCN (PubMed:27353360)

Research Field

Signal Transduction

Synonyms

AHSA1; C14orf3; HSPC322; Activator of 90 kDa heat shock protein ATPase homolog 1; AHA1; p38

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sf9-cell.html [Content Brief]