Mitofusin 1 Antibody (YA291)
(Synonyms: HR2; mKIAA4032; D3Ertd265e; 2310002F04Rik; 6330416C07Rik)Mitofusin 1 Antibody (YA291) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mitofusin 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Mouse, Rat, Hamster
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Formulation:
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Mitofusin 1 Antibody (YA291) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mitofusin 1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityMouse, Rat, Hamster
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Observed Molecular WeightObserved band size: 84 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 84 kDa
Entrez Gene: 67414 Mouse ; 192647 Rat
SwissProt: Q811U4 Mouse ; Q8R4Z9 Rat
Synthetic peptide corresponding to rat Mitofusin 1.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Mitofusin 1 is a Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity[1][2].
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Subcellular Localization
Mitochondrion outer membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Ubiquitous. In brain, it is expressed at weaker level than MFN2, while it is expressed at a higher level than MFN2 in heart and testis. Expressed at high level in elongating spermatids of seminiferous tubules -
Subunit
Homodimer, also in the absence of bound GTP. Forms higher oligomers in the presence of a transition state GTP analog (By similarity). Forms homomultimers and heteromultimers with MFN2 (PubMed:14561718). Oligomerization is essential for mitochondrion fusion. Component of a high molecular weight multiprotein complex (By similarity). Interacts with VAT1 (PubMed:17105775). Interacts with THG1L; THG1L probably functions as a guanyl-nucleotide exchange factor/GEF, activating MFN1
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SwissProt ID
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Synonyms
HR2; mKIAA4032; D3Ertd265e; 2310002F04Rik; 6330416C07Rik
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Research Field
Neuroscience
Documentation
[1]. Ishihara N, et al. Regulation of mitochondrial morphology by membrane potential, and DRP1-dependent division and FZO1-dependent fusion reaction in mammalian cells. Biochem Biophys Res Commun. 2003 Feb 21;301(4):891-8. [Content Brief]
[2]. Eura Y, et al. Two mitofusin proteins, mammalian homologues of FZO, with distinct functions are both required for mitochondrial fusion. J Biochem. 2003 Sep;134(3):333-44. [Content Brief]
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