MGMT Antibody (YA5548)
(Synonyms: Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT)MGMT Antibody (YA5548) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MGMT.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
MGMT Antibody (YA5548) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MGMT.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 22 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 22 kDa
Synthesized peptide derived from human MGMT AA range: 1-100
Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MGMT encodes O6-methylguanine-DNA methyltransferase, also called AGT, a DNA repair protein that removes alkyl groups from O6-alkylguanine adducts[1]. Mechanistically, MGMT repairs cytotoxic O6-methylguanine and O6-chloroethylguanine lesions induced by methylating or chloroethylating agents, thereby limiting DNA damage signaling and alkylating-drug cytotoxicity[2][3]. In glioma, MGMT promoter methylation silences MGMT expression and predicts clinical response to alkylating agents, including temozolomide[4][5]. Compared with separate isoform targets, the retrieved literature describes MGMT/AGT as the same repair protein, so isoform-specific claims require additional evidence[1]. For experimental applications, O6-benzylguanine and O6- (4-bromothenyl) guanine have been used to inactivate MGMT, but normal-tissue MGMT inhibition can increase alkylating-drug toxicity[6].
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Subcellular Localization
Nucleus
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SwissProt ID
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Synonyms
Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT
Documentation
[1]. Fang Q. The Versatile Attributes of MGMT: Its Repair Mechanism, et al. The Versatile Attributes of MGMT: Its Repair Mechanism, Crosstalk with Other DNA Repair Pathways, and Its Role in Cancer. Cancers (Basel). 2024 Jan 11;16(2):331. [Content Brief]
[2]. Kaina B, et al. MGMT: key node in the battle against genotoxicity, carcinogenicity and apoptosis induced by alkylating agents. DNA Repair (Amst). 2007 Aug 1;6(8):1079-99. [Content Brief]
[3]. Fan CH, et al. O6-methylguanine DNA methyltransferase as a promising target for the treatment of temozolomide-resistant gliomas. Cell Death Dis. 2013 Oct 24;4(10):e876. [Content Brief]
[4]. Esteller M, et al. Inactivation of the DNA-repair gene MGMT and the clinical response of gliomas to alkylating agents. N Engl J Med. 2000 Nov 9;343(19):1350-4. [Content Brief]
[5]. Hegi ME, et al. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med. 2005 Mar 10;352(10):997-1003. [Content Brief]
[6]. Kaina B, et al. Targeting O⁶-methylguanine-DNA methyltransferase with specific inhibitors as a strategy in cancer therapy. Cell Mol Life Sci. 2010 Nov;67(21):3663-81. [Content Brief]
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