Intestinal Alkaline Phosphatase Antibody (YA1559)

Cat. No.: HY-P81814: Data Sheet COA
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Intestinal Alkaline Phosphatase Antibody (YA1559) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Intestinal Alkaline Phosphatase.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Intestinal Alkaline Phosphatase Antibody (YA1559) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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제품 설명

Intestinal Alkaline Phosphatase Antibody (YA1559) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Intestinal Alkaline Phosphatase.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 57 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P09923

Gene ID

248 [NCBI]

Immunogen

A synthesized peptide derived from human inTinal alkaline phosphatase aa1-55/528.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Intestinal Alkaline Phosphatase Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Alkaline phosphatase that can hydrolyze various phosphate compounds

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor

Subunit：Homodimer

RRID

AB_3103914

Database

Entrez Gene: 248 Human

SwissProt: P09923 Human

OMIM: 171740 Human

Research Field

Tags & Cell Markers

Synonyms

Alpi; Glycerophosphatase; IAP

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Intestinal Alkaline Phosphatase Antibody (YA1559) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Intestinal Alkaline Phosphatase Antibody (YA1559)

Intestinal Alkaline Phosphatase Antibody (YA1559) Featured Recommendations:

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Intestinal Alkaline Phosphatase Antibody (YA1559) Related Classifications

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