NELFE Antibody (YA1365)
(Synonyms: RD; RDP; RDBP; D6S45; NELF-E)
Based on 1 Customer Validation
NELFE Antibody (YA1365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELFE.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:200-1:500 | 1:200-1:500 | 1:10-1:100 | 1:50-1:100 |
Product Details
NELFE Antibody (YA1365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELFE.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 43 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 43 kDa
Entrez Gene: 7936 Human ; 27632 Mouse ; 294258 Rat
SwissProt: P18615 Human ; P19426 Mouse ;
OMIM: 154040 Human
Recombinant protein of human NELFe
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NELFE is essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Tissue_specificity:Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas -
Isoforms & Post-Translational Modification
P18615 has 3 isomers: P18615-1: 43240 Da (predicted); P18615-3: 43925 Da (predicted); P18615-4: 40216 Da (predicted).
Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin;Sumoylated;Poly-ADP-ribosylated by PARP1, thereby preventing RNA-binding and relieving transcription pausing -
Subunit
The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE (PubMed:10199401, PubMed:27282391). Interacts with NELFB (By similarity)
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SwissProt ID
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Synonyms
RD; RDP; RDBP; D6S45; NELF-E
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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