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CTNNA3 Antibody (YA3001)

(Synonyms: Alpha T catenin; Cadherin associated protein; Catenin alpha 3; Catna3; CTNNA3; VR22)
Customer Review

Based on 1 Customer Validation

CTNNA3 Antibody (YA3001) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CTNNA3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • CTNNA3 Antibody (YA3001) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50

Product Details

Description

CTNNA3 Antibody (YA3001) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CTNNA3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 100 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CTNNA3 aa700-895/895.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105370

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), C2C12 (lane 2, 20 μg), C6 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), Mouse brain (lane 5, 20 μg), and Rat brain (lane 6, 20 μg) using CTNNA3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Immunohistochemical analysis of paraffin-embedded human rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    Immunohistochemical analysis of paraffin-embedded rat duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CTNNA3 may be involved in formation of stretch-resistant cell-cell adhesion complexes

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell junction, desmosome

  • Expression


    Tissue_specificity:Predominantly expressed in heart and testis. Expressed at lower levels in brain, kidney, liver and skeletal muscle

  • Isoforms & Post-Translational Modification

    Q9UI47 has 2 isomers: Q9UI47-1: 99809 Da (predicted); Q9UI47-2: 57332 Da (predicted).

  • Subunit

    Interacts with CTNNB1 (PubMed:11590244). Interacts with PKP2 (PubMed:17535849)

  • SwissProt ID

    Q9UI47

  • Gene ID
    29119 [NCBI]
  • Synonyms

    Alpha T catenin; Cadherin associated protein; Catenin alpha 3; Catna3; CTNNA3; VR22

  • Research Field

    Signal Transduction

CTNNA3 Antibody (YA3001) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), C2C12 (lane 2, 20 μg), C6 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), Mouse brain (lane 5, 20 μg), and Rat brain (lane 6, 20 μg) using CTNNA3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image
    1/16
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • CTNNA3 Antibody (YA3001) Product Image
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), C2C12 (lane 2, 20 μg), C6 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), Mouse brain (lane 5, 20 μg), and Rat brain (lane 6, 20 μg) using CTNNA3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • Experimental Validation Results for CTNNA3 Antibody (YA3001)
  • CTNNA3 Antibody (YA3001) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 20 μg), C2C12 (lane 2, 20 μg), C6 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), Mouse brain (lane 5, 20 μg), and Rat brain (lane 6, 20 μg) using CTNNA3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CTNNA3 Antibody (YA3001) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat duodenum tissue using CTNNA3 Antibody (HY-P83256, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CTNNA3 Antibody (YA3001) Product Image WB
  • CTNNA3 Antibody (YA3001) Product Image IHC-P
  • CTNNA3 Antibody (YA3001) Product Image IHC-P
  • CTNNA3 Antibody (YA3001) Product Image IHC-P
  • CTNNA3 Antibody (YA3001) Product Image IHC-P
  • CTNNA3 Antibody (YA3001) Product Image IHC-P
  • CTNNA3 Antibody (YA3001) Product Image IHC-P