VPS33B Antibody (YA8993)
(Synonyms: Vacuolar protein sorting-associated protein 33B, hVPS33B)
Based on 1 Customer Validation
VPS33B Antibody (YA8993) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VPS33B.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human, mouse, rat
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
VPS33B Antibody (YA8993) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VPS33B.
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Host Mouse
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Species Reactivityhuman, mouse, rat
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Calculated Molecular Weight Predicted band size: 71 kDa
OMIM: 608552
A synthesized peptide derived from human VPS33B.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
VPS33B may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity)[1][2][3].
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Subcellular Localization
Late endosome membrane; Lysosome membrane; Early endosome; Cytoplasmic vesicle, clathrin-coated vesicle; Recycling endosome
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Expression
Tissue_Specificity: Ubiquitous; highly expressed in testis and low expression in the lung. -
Isoforms & Post-Translational Modification
VPS33B has 2 isoforms, Q9H267-1: amino acid length is 617, molecular weight is 70585 Da (predicted); Q9H267-2: amino acid length is 526, molecular weight is 60032 Da (predicted).Phosphorylated on tyrosine residues
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Subunit
Interacts with RAB11A and VIPAS39.
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SwissProt ID
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Synonyms
Vacuolar protein sorting-associated protein 33B, hVPS33B
Documentation
[1]. Gruber R, et al. Autosomal Recessive Keratoderma-Ichthyosis-Deafness (ARKID) Syndrome Is Caused by VPS33B Mutations Affecting Rab Protein Interaction and Collagen Modification. J Invest Dermatol. 2017 Apr;137(4):845-854. [Content Brief]
[2]. Bach H, et al. Mycobacterium tuberculosis virulence is mediated by PtpA dephosphorylation of human vacuolar protein sorting 33B. Cell Host Microbe. 2008 May 15;3(5):316-22. [Content Brief]
[3]. Cullinane AR, et al. Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization. Nat Genet. 2010 Apr;42(4):303-12. [Content Brief]
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