eIF3g Antibody (YA6937)

Cat. No.: HY-P87249: Data Sheet User Guide for Antibodies
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eIF3g Antibody (YA6937) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF3g.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

eIF3g Antibody (YA6937) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF3g.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size：36 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O75821

Immunogen

Recombinant protein within human eIF3g aa 1-320.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:25000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:1000
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). This subunit can bind 18S rRNA|(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426)

Subcellular Localization：Cytoplasm,Nucleus,Cytoplasm, perinuclear region

Isoforms & Post-Translational Modification：O75821: 320 amino acids, molecular weight 35611 Da.

Subunit：Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M

Synonyms

44kDa antibody

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]