RPS27 Antibody
(Synonyms: MPS1, RPS27, Small ribosomal subunit protein eS27, 40S ribosomal protein S27, Metallopan-stimulin 1, MPS-1)RPS27 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RPS27.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
RPS27 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RPS27.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 9 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 9 kDa
Synthetic peptide corresponding to the N-term region of human RPS27.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RPS27 is a Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for proper rRNA processing and maturation of 18S rRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome[1][2][3][4].
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Subcellular Localization
Cytoplasm; Nucleus, nucleolus
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Expression
Tissue_Specificity: Expressed in a wide variety of actively proliferating cells and tumor tissues. -
Isoforms & Post-Translational Modification
RPS27 has an amino acid length of 84, molecular weight is 9461 Da.
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Subunit
Component of the small ribosomal subunit (Probable).
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SwissProt ID
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Synonyms
MPS1, RPS27, Small ribosomal subunit protein eS27, 40S ribosomal protein S27, Metallopan-stimulin 1, MPS-1
Documentation
[1]. Anger AM, et al. Structures of the human and Drosophila 80S ribosome. Nature. 2013 May 2;497(7447):80-5. [Content Brief]
[2]. Vladimirov SN, et al. Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry. Eur J Biochem. 1996 Jul 1;239(1):144-9. [Content Brief]
[3]. Wang R, et al. Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. Br J Haematol. 2015 Mar;168(6):854-64. [Content Brief]
[4]. Singh S, et al. Nucleolar maturation of the human small subunit processome. Science. 2021 Sep 10;373(6560):eabj5338. [Content Brief]
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