OAT1 Antibody (YA9007)
(Synonyms: OAT1, PAHT, SLC22A6, Solute carrier family 22 member 6, Organic anion transporter 1, PAH transporter, Renal organic anion transporter 1, hOAT1, hPAHT, hROAT1)
Based on 1 Customer Validation
OAT1 Antibody (YA9007) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OAT1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, IF-Tissue, IHC-P, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1:100-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
OAT1 Antibody (YA9007) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OAT1.
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Host Mouse
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Species Reactivityhuman
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Observed Molecular WeightObserved band size: 78 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 62 kDa
OMIM: 607582
A synthesized peptide derived from human OAT1.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
OAT1 is a Secondary active transporter that functions as a Na(+)-independent organic anion (OA)/dicarboxylate antiporter where the uptake of one molecule of OA into the cell is coupled with an efflux of one molecule of intracellular dicarboxylate such as 2-oxoglutarate or glutarate. Mediates the uptake of OA across the basolateral side of proximal tubule epithelial cells, thereby contributing to the renal elimination of endogenous OA from the systemic circulation into the urine. Functions as a biopterin transporters involved in the uptake and the secretion of coenzymes tetrahydrobiopterin (BH4), dihydrobiopterin (BH2) and sepiapterin to urine, thereby determining baseline levels of blood biopterins. Transports prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) and may contribute to their renal excretion. Also mediates the uptake of cyclic nucleotides such as cAMP and cGMP. Involved in the transport of neuroactive tryptophan metabolites kynurenate (KYNA) and xanthurenate (XA) and may contribute to their secretion from the brain. May transport glutamate. Also involved in the disposition of uremic toxins and potentially toxic xenobiotics by the renal organic anion secretory pathway, helping reduce their undesired toxicological effects on the body. Uremic toxins include the indoxyl sulfate (IS), hippurate/N-benzoylglycine (HA), indole acetate (IA), 3-carboxy-4- methyl-5-propyl-2-furanpropionate (CMPF) and urate. Xenobiotics include the mycotoxin ochratoxin (OTA). May also contribute to the transport of organic compounds in testes across the blood-testis-barrier[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Basolateral cell membrane; Basal cell membrane
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Expression
Tissue_Specificity: Strongly expressed in kidney. Expressed at lower level in liver, skeletal muscle, brain and placenta. In kidney, found at the basolateral membrane of the proximal tubule. In testis, primarily localized to the basal membrane of Sertoli cells and weakly expressed in Leydig cells and vascular endothelial cells. -
Isoforms & Post-Translational Modification
OAT1 has 4 isoforms, Q4U2R8-1: amino acid length is 563, molecular weight is 61816 Da (predicted); Q4U2R8-2: amino acid length is 550, molecular weight is 60318 Da (predicted); Q4U2R8-3: amino acid length is 506, molecular weight is 55858 Da (predicted); Q4U2R8-4: amino acid length is 519, molecular weight is 57357 Da (predicted).
Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane -
SwissProt ID
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Synonyms
OAT1, PAHT, SLC22A6, Solute carrier family 22 member 6, Organic anion transporter 1, PAH transporter, Renal organic anion transporter 1, hOAT1, hPAHT, hROAT1
Documentation
[1]. Jung KY, et al. Characterization of ochratoxin A transport by human organic anion transporters. Life Sci. 2001 Sep 21;69(18):2123-35. [Content Brief]
[2]. Kimura H, et al. Human organic anion transporters and human organic cation transporters mediate renal transport of prostaglandins. J Pharmacol Exp Ther. 2002 Apr;301(1):293-8. [Content Brief]
[3]. Deguchi T, et al. Characterization of uremic toxin transport by organic anion transporters in the kidney. Kidney Int. 2004 Jan;65(1):162-74. [Content Brief]
[4]. Uwai Y, et al. Interaction and transport of kynurenic acid via human organic anion transporters hOAT1 and hOAT3. Pharmacol Res. 2012 Feb;65(2):254-60. [Content Brief]
[5]. Uwai Y, et al. Transport of xanthurenic acid by rat/human organic anion transporters OAT1 and OAT3. Biosci Biotechnol Biochem. 2013;77(7):1517-21. [Content Brief]
[6]. Ohashi A, et al. Organic anion transporters, OAT1 and OAT3, are crucial biopterin transporters involved in bodily distribution of tetrahydrobiopterin and exclusion of its excess. Mol Cell Biochem. 2017 Nov;435(1-2):97-108. [Content Brief]
[7]. Lu R, et al. Cloning of the human kidney PAH transporter: narrow substrate specificity and regulation by protein kinase C. Am J Physiol. 1999 Feb;276(2):F295-303. [Content Brief]
[8]. Hosoyamada M, et al. Molecular cloning and functional expression of a multispecific organic anion transporter from human kidney. Am J Physiol. 1999 Jan;276(1):F122-8. [Content Brief]
[9]. Henjakovic M, et al. Human organic anion transporter 2 is distinct from organic anion transporters 1 and 3 with respect to transport function. Am J Physiol Renal Physiol. 2015 Nov 15;309(10):F843-51. [Content Brief]
[10]. Hau RK, et al. Localization of Xenobiotic Transporters Expressed at the Human Blood-Testis Barrier. Drug Metab Dispos. 2022 Jun;50(6):770-780. [Content Brief]
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