Salivary alpha Amylase Antibody (YA3739)

(Synonyms: AMY1)
Customer Review

Based on 1 Customer Validation

Salivary alpha Amylase Antibody (YA3739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Salivary alpha Amylase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

Salivary alpha Amylase Antibody (YA3739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Salivary alpha Amylase.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57.8 kDa
Species Reactivity Database
Immunogen

Purified recombinant fragment of human AMY1A (AA: 172-284) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718819

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Salivary alpha Amylase Antibody (YA3739)
    Western blot analysis of extracts from SiHa(lane 2(20μg) ,PANC-1(lane 3(20μg) ,Rat Liver(lane 4(20μg)and Rat Heart(lane 5(20μg) using Salivary alpha Amylase Antibody(HY-P84042).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993,1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Salivary alpha Amylase is a P0DUB6: Calcium-binding enzyme that initiates starch digestion in the oral cavity. Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins
    P0DTE7: Calcium-binding enzyme that initiates starch digestion in the oral cavity. Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins
    P0DTE8: Calcium-binding enzyme that initiates starch digestion in the oral cavity. Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins
    [1].

  • Subcellular Localization

    P0DUB6: Secreted
    P0DTE7: Secreted
    P0DTE8: Secreted

  • Subunit

    P0DUB6: Monomer
    P0DTE7: Monomer
    P0DTE8: Monomer

  • SwissProt ID

    P0DUB6

  • Gene ID
    276 [NCBI]
  • Synonyms

    AMY1

Salivary alpha Amylase Antibody (YA3739) Related Classifications

MOQ
Minimum order quantity
100 mg

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