Salivary alpha Amylase Antibody (YA3739)

Cat. No.: HY-P84042: Ficha de datos COA
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Salivary alpha Amylase Antibody (YA3739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Salivary alpha Amylase.

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Tamaño	Stock	Cantidad
Muestra gratis	Apply now
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Salivary alpha Amylase Antibody (YA3739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Salivary alpha Amylase.

Host

Mouse

Clonality

Monoclonal Antibody

Peso molecular

Predicted band size: 57.8 kDa;

Observed band size: 58 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P0DUB6

Gene ID

276 [NCBI]

Immunogen

Purified recombinant fragment of human AMY1A (AA: 172-284) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from SiHa(lane 2(20μg) ,PANC-1(lane 3(20μg) ,Rat Liver(lane 4(20μg)and Rat Heart(lane 5(20μg) using Salivary alpha Amylase Antibody（HY-P84042）.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004，1/10,000) was used for 1 hour at room temperature.

Background

Function：P0DUB6: Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308)
P0DTE7: Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308)
P0DTE8: Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4) -alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308)

Subcellular Localization：P0DUB6: Secreted
P0DTE7: Secreted
P0DTE8: Secreted

Subunit：P0DUB6: Monomer
P0DTE7: Monomer
P0DTE8: Monomer

RRID

AB_3718819

Database

SwissProt: P0DTE7/P0DUB6/P0DTE8

Synonyms

AMY1

Documentación

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Ficha de datos (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)