NFAT5 Antibody (YA5391)
(Synonyms: Nuclear factor of activated T-cells 5; NF-AT5; T-cell transcription factor NFAT5; Tonicity-responsive enhancer-binding protein; TonE-binding protein; TonEBP; )
Based on 1 Customer Validation
NFAT5 Antibody (YA5391) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to NFAT5.
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Host:
Mouse
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Application:
IHC-P, WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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|---|---|---|
| Dilution Ratio | 1:50-200 | 1:1000-2000 |
Product Details
NFAT5 Antibody (YA5391) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to NFAT5.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 200 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Synthesized peptide derived from human NFAT5
affinity chromatography.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NFAT5 transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA[1][2][3][4].
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Subcellular Localization
Nucleus; Cytoplasm; Chromosome
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Expression
Tissue_specificity:Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes -
Isoforms & Post-Translational Modification
O94916 has 5 isomers: O94916-1: 165763 Da (predicted); O94916-2: 157983 Da (predicted); O94916-3: 10459 Da (predicted); O94916-4: 167737 Da (predicted); O94916-5: 167808 Da (predicted).
Phosphorylated (PubMed:10377394). Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization (PubMed:21209322);Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage -
Subunit
Homodimer when bound to DNA, completely encircles its DNA target (PubMed:11780147). Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Does not bind with Fos and Jun transcription factors (PubMed:10377394). Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation (PubMed:22266867)
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SwissProt ID
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Synonyms
Nuclear factor of activated T-cells 5; NF-AT5; T-cell transcription factor NFAT5; Tonicity-responsive enhancer-binding protein; TonE-binding protein; TonEBP;
Documentation
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Data Sheet (232 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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