NFAT5 Antibody (YA5391)

Cat. No.: HY-P85699: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

NFAT5 Antibody (YA5391) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to NFAT5.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NFAT5 Antibody (YA5391) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to NFAT5.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 200 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O94916

Gene ID

10725 [NCBI]

Immunogen

Synthesized peptide derived from human NFAT5

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
WB WB: Western Blot	1:1000-2000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394). Mediates the transcriptional response to hypertonicity (PubMed:10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:34049076)

Subcellular Localization：Nucleus; Cytoplasm; Chromosome

Expression：
Tissue_specificity:Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes

Isoforms & Post-Translational Modification：O94916 has 5 isomers: O94916-1: 165763 Da (predicted); O94916-2: 157983 Da (predicted); O94916-3: 10459 Da (predicted); O94916-4: 167737 Da (predicted); O94916-5: 167808 Da (predicted).
Phosphorylated (PubMed:10377394). Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization (PubMed:21209322);Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage

Subunit：Homodimer when bound to DNA, completely encircles its DNA target (PubMed:11780147). Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Does not bind with Fos and Jun transcription factors (PubMed:10377394). Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation (PubMed:22266867)

Synonyms

Nuclear factor of activated T-cells 5; NF-AT5; T-cell transcription factor NFAT5; Tonicity-responsive enhancer-binding protein; TonE-binding protein; TonEBP;

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)