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Inhibin beta A Antibody (YA2780)

(Synonyms: INHBA; Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein; EDF)
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Based on 1 Customer Validation

Inhibin beta A Antibody (YA2780) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Inhibin beta A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Inhibin beta A Antibody (YA2780)
  • Inhibin beta A Antibody (YA2780) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Inhibin beta A Antibody (YA2780) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Inhibin beta A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Inhibin beta A aa55-100.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105023

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Inhibin beta A Antibody (YA2780)
    Western blot analysis of extracts from A549 (lane2, 20μg), HepG2 (lane3, 20μg), PANC-1 (lane4, 20μg) and Rat heart tissue (lane5, 20μg) using Inhibin beta A Antibody (HY-P83035). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Inhibin beta A is an Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition; Activin A is a homodimer of INHBA that plays a role in several essential biological processes including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Signals through type I (such as ACVR1B or ACVR1C) and type II receptors (such as ACVR2A, ACVR2B or BMPR2) which, upon ligand binding, phosphorylate SMAD2 and SMAD3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and modulate gene expression. Can also activate alternative non-canonical intracellular signaling pathways including the p38 MAPK, extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNKs) to modulate cell migration and differentiation. Alternatively, promotes osteoblastic differentiation via ACVRL1-SMAD1/5/9 pathway. In addition, can engage the type I receptor ACVR1 to form an ACVR1-activin A-type II receptor non-signaling complex (NSC) that renders receptors unavailable for engagement with BMPs, hence resulting in an apparent inhibition of ACVR1-mediated BMP signaling; Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor[1][2][3][4][5][6].

  • Subcellular Localization

    Secreted

  • Subunit

    Dimeric, linked by one or more disulfide bonds. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA. Activin A is a homodimer of beta-A/INHBA. Activin AB is a dimer of beta-A/INHBA and beta-B/INHBB. Interacts with FST and FSTL3; these interactions prevent activin A interaction to its type II receptor (PubMed:11279126). Activin A interacts with ACVR2A (PubMed:10652306). Activin A interacts with BMPR2 (PubMed:24018044). Inhibin A interacts with ACVR1; this interaction creates a non-signaling complex (NSC) that inhibits ACVR1-mediated BMP signaling (PubMed:10652306). Inhibin A interacts with ACVR2A (PubMed:10652306)

  • SwissProt ID

    P08476

  • Gene ID
    3624 [NCBI]
  • Synonyms

    INHBA; Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein; EDF

  • Research Field

    Signal Transduction

Inhibin beta A Antibody (YA2780) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2, 20μg), HepG2 (lane3, 20μg), PANC-1 (lane4, 20μg) and Rat heart tissue (lane5, 20μg) using Inhibin beta A Antibody (HY-P83035). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Inhibin beta A Antibody (YA2780) Product Image
    1/16
  • Experimental Validation Results for Inhibin beta A Antibody (YA2780)
  • Inhibin beta A Antibody (YA2780) Product Image
  • Experimental Validation Results for Inhibin beta A Antibody (YA2780)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2, 20μg), HepG2 (lane3, 20μg), PANC-1 (lane4, 20μg) and Rat heart tissue (lane5, 20μg) using Inhibin beta A Antibody (HY-P83035). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Inhibin beta A Antibody (YA2780)
  • Inhibin beta A Antibody (YA2780) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane2, 20μg), HepG2 (lane3, 20μg), PANC-1 (lane4, 20μg) and Rat heart tissue (lane5, 20μg) using Inhibin beta A Antibody (HY-P83035). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Inhibin beta A Antibody (YA2780) Product Image WB