ATIC Antibody (YA9768)
(Synonyms: ATIC, 5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase, PURH, IMPCHASE, AICARFT, Phosphoribosylaminoimidazolecarboxamide Formyltransferase/IMP Cyclohydrolase, AICAR Transformylase/Inosine Monophosphate Cyclohydrolase, Bifunctional Purine Biosynthesis Protein ATIC, 5-Aminoimidazole-4-Carboxamide-1-Beta-D-Ribonucleotide Transformylase/Inosinicase, AICAR Formyltransferase/IMP Cyclohydrolase Bifunctional Enzyme, Epididymis Secretory Sperm Binding Protein Li 70p, Bifunctional Purine Biosynthesis Protein PURH, AICARFT/IMPCHASE, HEL-S-70p, AICAR)ATIC Antibody (YA9768) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to ATIC.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB, FC, ICC/IF
-
Reactivity :
Human, mouse, rat
-
Formulation:
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
FC
FC: Flow Cytometry
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:200-2000 | 1:100-1000 |
Product Details
ATIC Antibody (YA9768) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to ATIC.
-
Host Mouse
-
Species ReactivityHuman, mouse, rat
-
Observed Molecular WeightObserved band size: 65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 65 kDa,
Recombinant protein of human ATIC
affinity purified.
Non-conjugated
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
ATIC is a Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias. Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization[1][2][3][4][5].
-
Subcellular Localization
Cytoplasm, cytosol
-
Expression
Tissue_Specificity: Present in the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas. -
Isoforms & Post-Translational Modification
ATIC has 2 isoforms, P31939-1: amino acid length is 592, molecular weight is 64616 Da (predicted); P31939-2: amino acid length is 591, molecular weight is 64524 Da (predicted).
-
Subunit
Homodimer.
-
SwissProt ID
-
Synonyms
ATIC, 5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase, PURH, IMPCHASE, AICARFT, Phosphoribosylaminoimidazolecarboxamide Formyltransferase/IMP Cyclohydrolase, AICAR Transformylase/Inosine Monophosphate Cyclohydrolase, Bifunctional Purine Biosynthesis Protein ATIC, 5-Aminoimidazole-4-Carboxamide-1-Beta-D-Ribonucleotide Transformylase/Inosinicase, AICAR Formyltransferase/IMP Cyclohydrolase Bifunctional Enzyme, Epididymis Secretory Sperm Binding Protein Li 70p, Bifunctional Purine Biosynthesis Protein PURH, AICARFT/IMPCHASE, HEL-S-70p, AICAR
Documentation
[1]. Bulock KG, et al. The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling. J Biol Chem. 2002 Jun 21;277(25):22168-74. [Content Brief]
[2]. Vergis JM, et al. Catalytic mechanism of the cyclohydrolase activity of human aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase. Biochemistry. 2004 Feb 10;43(5):1184-92. [Content Brief]
[3]. Wall M, et al. Human AICAR transformylase: role of the 4-carboxamide of AICAR in binding and catalysis. Biochemistry. 2000 Sep 19;39(37):11303-11. [Content Brief]
[4]. Sugita T, et al. Characterization of molecularly cloned human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase. J Biochem. 1997 Aug;122(2):309-13. [Content Brief]
[5]. Boutchueng-Djidjou M, et al. The last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) plays a central role in insulin signaling and the Golgi/endosomes protein network. Mol Cell Proteomics. 2015 Apr;14(4):1079-92. [Content Brief]
Powered by Bioz
