Copy Link Print Information

PKA R2/PKR2 Antibody (YA132)

Revisión del cliente

Based on 1 Customer Validation

PKA R2/PKR2 Antibody (YA132) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA R2/PKR2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PKA R2/PKR2 Antibody (YA132)
  • PKA R2/PKR2 Antibody (YA132) Product Image
Tamaño
Precio
Stock
Cantidad
Amount: USD 0.00
Please select quantity
or
or

* Seleccione Cantidad antes de agregar artículos.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:10-1:50

Product Details

Descripciòn

PKA R2/PKR2 Antibody (YA132) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA R2/PKR2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database

Entrez Gene: 5576 Human

SwissProt: P13861 Human

Immunogen

Synthetic peptide corresponding to Human PKA R2.AA range:272-404.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102512

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Envío

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PKA R2/PKR2 Antibody (YA132)
    Western blot analysis of extracts from K562(lane 2(40μg) ) ,Hela(lane 3(40μg) )and Jurkat(lane 4(40μg) ) using PKA R2 (HY-P80429) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80429, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PKA R2/PKR2 is a Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:There are four known types of regulatory chains: I-α, I-β, II-α, and II-β. Their expression varies in different tissues; some are constitutively expressed, while others are inducibly expressed.

  • Isoforms & Post-Translational Modification

    P13861 has 2 isomers: P13861-1: 45518 Da (predicted); P13861-2: 43067 Da (predicted).
    Phosphorylated by the activated catalytic chain

  • Subunit

    The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains.

  • SwissProt ID

    P13861

  • Gene ID
    5576 [NCBI]
  • Research Field

    Signal Transduction

PKA R2/PKR2 Antibody (YA132) Related Classifications

MOQ
Minimum order quantity
100 mg

Obtener un presupuesto En stock

Other size
Obtener un presupuesto
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(40μg) ) ,Hela(lane 3(40μg) )and Jurkat(lane 4(40μg) ) using PKA R2 (HY-P80429) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80429, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • PKA R2/PKR2 Antibody (YA132) Product Image
    1/16
  • Experimental Validation Results for PKA R2/PKR2 Antibody (YA132)
  • PKA R2/PKR2 Antibody (YA132) Product Image
  • Experimental Validation Results for PKA R2/PKR2 Antibody (YA132)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(40μg) ) ,Hela(lane 3(40μg) )and Jurkat(lane 4(40μg) ) using PKA R2 (HY-P80429) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80429, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PKA R2/PKR2 Antibody (YA132)
  • PKA R2/PKR2 Antibody (YA132) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(40μg) ) ,Hela(lane 3(40μg) )and Jurkat(lane 4(40μg) ) using PKA R2 (HY-P80429) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80429, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • PKA R2/PKR2 Antibody (YA132) Product Image WB