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SLP76 Antibody (YA6981)

(Synonyms: CG8697 antibody; LCP 2 antibody; LCP2 antibody; LCP2_HUMAN antibody; Lymphocyte cytosolic protein 2 antibody; SH2 domain containing leukocyte protein 76 KD antibody; SH2 domain containing leukocyte protein of 76kD antibody; SH2 domain containing leukocyte protein of 76kDa antibody; SH2 domain-containing leukocyte protein of 76 kDa antibody; SLP 76 antibody)
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Based on 1 Customer Validation

SLP76 Antibody (YA6981) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLP76.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SLP76 Antibody (YA6981)
  • SLP76 Antibody (YA6981) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:5000 1:100 1-2μg/sample

Product Details

Description

SLP76 Antibody (YA6981) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLP76.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Immunogen

Recombinant protein within human SLP76 aa 1- 533/533

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLP76 Antibody (YA6981)
    Western blot analysis of extracts from Jurkat (lane 1(20μg)) and THP-1 (lane 2(20μg)) using SLP76 Antibody (HY-P87298) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    SLP76 is an Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells. Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK. In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation. Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_Specificity: Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines

  • Isoforms & Post-Translational Modification

    Q13094: 533 amino acids, molecular weight 60188 Da.

  • Subunit

    Interacts with SLA

  • SwissProt ID

    Q13094

  • Synonyms

    CG8697 antibody; LCP 2 antibody; LCP2 antibody; LCP2_HUMAN antibody; Lymphocyte cytosolic protein 2 antibody; SH2 domain containing leukocyte protein 76 KD antibody; SH2 domain containing leukocyte protein of 76kD antibody; SH2 domain containing leukocyte protein of 76kDa antibody; SH2 domain-containing leukocyte protein of 76 kDa antibody; SLP 76 antibody

SLP76 Antibody (YA6981) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1(20μg)) and THP-1 (lane 2(20μg)) using SLP76 Antibody (HY-P87298) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SLP76 Antibody (YA6981) Product Image
    1/16
  • Experimental Validation Results for SLP76 Antibody (YA6981)
  • SLP76 Antibody (YA6981) Product Image
  • Experimental Validation Results for SLP76 Antibody (YA6981)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1(20μg)) and THP-1 (lane 2(20μg)) using SLP76 Antibody (HY-P87298) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLP76 Antibody (YA6981)
  • SLP76 Antibody (YA6981) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 1(20μg)) and THP-1 (lane 2(20μg)) using SLP76 Antibody (HY-P87298) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • SLP76 Antibody (YA6981) Product Image WB