SLP76 Antibody (YA6981)

Cat. No.: HY-P87298: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

SLP76 Antibody (YA6981) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLP76.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SLP76 Antibody (YA6981) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLP76.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 60 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13094

Immunogen

Recombinant protein within human SLP76 aa 1- 533/533

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IP IP: Immunoprecipitation	1-2μg/sample

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat (lane 1(20μg)) and THP-1 (lane 2(20μg)) using SLP76 Antibody (HY-P87298) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed:11313406, PubMed:33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed:8673706, PubMed:8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed:11313406). Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed:33436632)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines

Isoforms & Post-Translational Modification：Q13094: 533 amino acids, molecular weight 60188 Da.

Subunit：Interacts with SLA

Synonyms

CG8697 antibody; LCP 2 antibody; LCP2 antibody; LCP2_HUMAN antibody; Lymphocyte cytosolic protein 2 antibody; SH2 domain containing leukocyte protein 76 KD antibody; SH2 domain containing leukocyte protein of 76kD antibody; SH2 domain containing leukocyte protein of 76kDa antibody; SH2 domain-containing leukocyte protein of 76 kDa antibody; SLP 76 antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)