Lipocalin 2 Antibody (YA992)

Cat. No.: HY-P81284A: Data Sheet COA
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User Guide for Antibodies
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Lipocalin 2 Antibody (YA992) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lipocalin 2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Lipocalin 2 Antibody (YA992) Featured Recommendations:

1 Publications Citing Use of MCE Lipocalin 2 Antibody (YA992)

•Int Immunopharmacol. 2025 Sep 23:162:115171. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Lipocalin 2 Antibody (YA992) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lipocalin 2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 23 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P80188

Gene ID

3934 [NCBI]

Immunogen

A synthesized peptide derived from human Lipocalin-2 aa50-100.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of A549 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A431 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413, PubMed:20581821, PubMed:27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:27780864). Can also bind siderophores from M.tuberculosis (PubMed:15642259, PubMed:21978368)

Subcellular Localization：Secreted; Cytoplasmic granule lumen; Cytoplasmic vesicle lumen

Expression：
Tissue_specificity:Detected in neutrophils (protein level) (PubMed:7683678, PubMed:8298140) . Expressed in bone marrow and tissues susceptible to microbial infection (PubMed:9339356) . Highly expressed in bone marrow, as well as in the uterus, prostate, salivary glands, stomach, appendix, colon, trachea, and lungs (PubMed:9339356) . Expressed in renal medullary tubules (PubMed:30418175) . Not detected in small intestine or peripheral blood leukocytes (PubMed:9339356) .

Induction:Expression is activated by the oncoprotein BCR-ABL; BCR-ABL misregulates expression via the JAK/STAT pathway and binding of STAT5A to the promoter (PubMed:19229297) . Induced by insulin (PubMed:30418175)

Isoforms & Post-Translational Modification：P80188 has 2 isomers: P80188-1: 22588 Da (predicted); P80188-2: 22457 Da (predicted).

Subunit：Monomer (PubMed:1281792, PubMed:7683678). Homodimer; disulfide-linked (PubMed:7683678). Heterodimer; disulfide-linked with MMP9 (PubMed:7683678)

RRID

AB_3103125

Database

Entrez Gene: 3934 Human

SwissProt: P80188 Human

OMIM: 600181 Human

Research Field

Immunology

Synonyms

LCN2; 24p3; MSFI; NGAL; Lipocalin-2; p25; Siderocalin; SV40 induced 24P3 protein; Uterocalin

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Lipocalin 2 Antibody (YA992) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Lipocalin 2 Antibody (YA992)

Lipocalin 2 Antibody (YA992) Featured Recommendations:

1 Publications Citing Use of MCE Lipocalin 2 Antibody (YA992)

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Background

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Lipocalin 2 Antibody (YA992) Related Classifications

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