Copy Link Print Information

Lipocalin 2 Antibody (YA992)

(Synonyms: LCN2; 24p3; MSFI; NGAL; Lipocalin-2; p25; Siderocalin; SV40 induced 24P3 protein; Uterocalin)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Lipocalin 2 Antibody (YA992) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lipocalin 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P ICC
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Lipocalin 2 Antibody (YA992) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Lipocalin 2 Antibody (YA992)

More

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Lipocalin 2 Antibody (YA992) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lipocalin 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Species Reactivity Database

Entrez Gene: 3934 Human

SwissProt: P80188 Human

Immunogen

A synthesized peptide derived from human Lipocalin-2 aa50-100.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103125

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded rat colon tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunocytochemistry analysis of A549 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    Immunocytochemistry analysis of A431 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Lipocalin 2 is an Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis[1][2][3][4][5].

  • Subcellular Localization

    Secreted; Cytoplasmic granule lumen; Cytoplasmic vesicle lumen

  • Expression


    Tissue_specificity:Detected in neutrophils (protein level) (PubMed:7683678, PubMed:8298140) . Expressed in bone marrow and tissues susceptible to microbial infection (PubMed:9339356) . Highly expressed in bone marrow, as well as in the uterus, prostate, salivary glands, stomach, appendix, colon, trachea, and lungs (PubMed:9339356) . Expressed in renal medullary tubules (PubMed:30418175) . Not detected in small intestine or peripheral blood leukocytes (PubMed:9339356) .

    Induction:Expression is activated by the oncoprotein BCR-ABL; BCR-ABL misregulates expression via the JAK/STAT pathway and binding of STAT5A to the promoter (PubMed:19229297) . Induced by insulin (PubMed:30418175)

  • Isoforms & Post-Translational Modification

    P80188 has 2 isomers: P80188-1: 22588 Da (predicted); P80188-2: 22457 Da (predicted).

  • Subunit

    Monomer (PubMed:1281792, PubMed:7683678). Homodimer; disulfide-linked (PubMed:7683678). Heterodimer; disulfide-linked with MMP9 (PubMed:7683678)

  • SwissProt ID

    P80188

  • Gene ID
    3934 [NCBI]
  • Synonyms

    LCN2; 24p3; MSFI; NGAL; Lipocalin-2; p25; Siderocalin; SV40 induced 24P3 protein; Uterocalin

  • Research Field

    Immunology

References

[1]. Yang J, et al. An iron delivery pathway mediated by a lipocalin. Mol Cell. 2002 Nov;10(5):1045-56. [Content Brief]

[2]. Bao G, et al. Iron traffics in circulation bound to a siderocalin (Ngal)-catechol complex. Nat Chem Biol. 2010 Aug;6(8):602-9. [Content Brief]

[3]. Shields-Cutler RR, et al. Human Metabolome-derived Cofactors Are Required for the Antibacterial Activity of Siderocalin in Urine. J Biol Chem. 2016 Dec 9;291(50):25901-25910. [Content Brief]

[4]. Holmes MA, et al. Siderocalin (Lcn 2) also binds carboxymycobactins, potentially defending against mycobacterial infections through iron sequestration. Structure. 2005 Jan;13(1):29-41. [Content Brief]

[5]. Hoette TM, et al. Immune interference in Mycobacterium tuberculosis intracellular iron acquisition through siderocalin recognition of carboxymycobactins. ACS Chem Biol. 2011 Dec 16;6(12):1327-31. [Content Brief]

View More

Lipocalin 2 Antibody (YA992) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat colon tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Lipocalin 2 Antibody (YA992) Product Image
    1/16
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Lipocalin 2 Antibody (YA992) Product Image
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat colon tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Experimental Validation Results for Lipocalin 2 Antibody (YA992)
  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat colon tissue using Lipocalin 2 Antibody (HY-P81284A, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using Lipocalin 2 Antibody (HY-P81284A, 1/50). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Lipocalin 2 Antibody (YA992) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Lipocalin 2 Antibody (YA992) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Lipocalin 2 with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lipocalin 2 Antibody (HY-P81284A) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image IHC-P
  • Lipocalin 2 Antibody (YA992) Product Image ICC
  • Lipocalin 2 Antibody (YA992) Product Image ICC